响应面优化水代法提取紫苏籽油及对运动大鼠免疫功能的影响  

Response surface methodology optimization of water extraction of perilla seed oil and its effect on immune function of exercise rats

作　　者：徐龙[1] 潘文文 崔洁 XU Long;PAN Wen-wen;CUI Jie(Qiqihar University,Qiqihar 161000,Heilongjiang,China;Harbin Institute of Technology,Harbin 150086,Heilongjiang,China)

机构地区：[1]齐齐哈尔大学,黑龙江齐齐哈尔161000 [2]哈尔滨工业大学,黑龙江哈尔滨150086

出　　处：《粮食与油脂》2021年第3期53-57,61,共6页Cereals & Oils

基　　金：2019年黑龙江省教育厅基本科研业务项目(135409235);2019年黑龙江省哲学社会科学研究项目(19TYC157)。

摘　　要：采用响应面优化水代法提取紫苏籽油的工艺。结果表明:最佳工艺条件为液料比8.4∶1(mL/g)、提取温度54℃、pH 5,此条件下紫苏籽油平均提取率为32.96%。大鼠试验表明,紫苏籽油可以改善由运动导致的脾脏和胸腺指数降低带来的免疫功能的影响,增强机体的巨噬细胞吞噬功能,具有免疫增强作用,并且量效关系明显。紫苏籽油可以改善由运动引起的血清溶血数量的降低,提高免疫器官指数,具有提高运动大鼠体液免疫功能的作用,表明紫苏籽油对运动大鼠免疫功能具有积极的影响。The response surface method was used to optimize the condition of perilla seed oil extracted by water substitution method.The results showed that the optimal process conditions were liquid-to-material ratio 8.4∶1(mL/g),extraction temperature 54℃,pH=5,and the average extraction rate of perilla seed oil under these conditions was 32.96%.Experiments in rats have shown that perilla seed oil can improve the immune function caused by an exercise-induced decrease in spleen and thymus index,enhance the phagocytic function of macrophages of the body,and have immune enhancement effects with obvious dose-effect relationship.Perilla seed oil can improve the reduction in the amount of serum hemolysis caused by exercise,increase the index of immune organs,and improve the humoral immune function of exercise rats,which indicating that perilla seed oil has a positive effect on the immune function of exercise rats.

关 键 词：紫苏籽油 力竭运动 免疫功能 水代法 

分 类 号：TS225.19[轻工技术与工程—粮食、油脂及植物蛋白工程]