Controlling the strontium-doping in calcium phosphate microcapsules through yeastregulated biomimetic mineralization  

作　　者：Miaojun Huang Tianjie Li Ting Pan Naru Zhao Yongchang Yao Zhichen Zhai Jiaan Zhou Chang Du Yingjun Wang 

机构地区：[1]School of Materials Science and Engineering,South China University of Technology,Guangzhou 510641,China [2]National Engineering Research Center for Tissue Restoration and Reconstruction,Guangzhou 510006,China [3]Ministry of Education Key Laboratory of Biomedical Materials Science and Engineering,Guangzhou 510006,China

出　　处：《Regenerative Biomaterials》2016年第5期269-276,共8页再生生物材料（英文版）

基　　金：This work was supported by National Basic Research Program of China(2012CB619100);National Natural Science Foundation of China(51072056,51572087);the 111 Project(B13039);Key grant of Chinese Ministry of Education(313022);Program for Changjiang Scholars and Innovative Research Team in University(IRT 0919).

摘　　要：Yeast cells have controllable biosorption on metallic ions during metabolism.However,few studies were dedicated to using yeast-regulated biomimetic mineralization process to control the strontium-doped positions in calcium phosphate microcapsules.In this study,the yeast cells were allowed to pre-adsorb strontium ions metabolically and then served as sacrificing template for the precipitation and calcination of mineral shell.The pre-adsorption enabled the microorganism to enrich of strontium ions into the inner part of the microcapsules,which ensured a slow-release profile of the trace element from the microcapsule.The co-culture with human marrow stromal cells showed that gene expressions of alkaline phosphatase and Collagen-I were promoted.The promotion of osteogenic differentiation was further confirmed in the 3D culture of cell-material complexes.The strategy using living microorganism as‘smart doping apparatus’to control incorporation of trace element into calcium phosphate paved a pathway to new functional materials for hard tissue regeneration.

关 键 词：calcium phosphate YEAST BIOTEMPLATE STRONTIUM 

分 类 号：R73[医药卫生—肿瘤]