An enzyme-loaded reactor using metal-organic framework-templated polydopamine microcapsule  

作　　者：Jing Wang Yongqin Lv 

机构地区：[1]Beijing Key Laboratory of Bioprocess,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China

出　　处：《Chinese Journal of Chemical Engineering》2021年第1期317-325,共9页中国化学工程学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31961133004, 21861132017);the National Key Research and Development Program of China (2018YFA0902200);the Fundamental Research Funds for the Central Universities (PT1917, buctrc201)。

摘　　要：Ultrathin polydopamine microcapsules with hierarchical structure and porosity were prepared for the immobilization of multienzymes using metal-organic framework(MOF) as the template.The multienzyme/MOF composite was first prepared using a "one-pot" co-precipitation approach via the coordination and self-assembly of zinc ions and 2-methylimidazole in the presence of enzymes.The obtained nanoparticles were then coated with polydopamine thin layer through the self-polymerization of dopamine under alkaline condition.The polydopamine microcapsules with an ultrathin shell thickness of ~48 nm were finally generated by removing the MOF template at acidic condition.Three enzymes were encapsulated in PDA microcapsules including carbonic anhydrase(CA),formate dehydrogenase(FateDH),and glutamate dehydrogenase(GDH).FateDH that catalyzed the main reaction of CO_(2) reduction to formic acid retained 94.7% activity of equivalent free FateDH.Compared with free multienzymes,the immobilized ones embedded in PDA microcapsules exhibited 4.5-times higher of formate production and high catalytic efficiency with a co-factor-based formate yield of 342%.

关 键 词：BIOCATALYSIS ENZYME IMMOBILIZATION MICROCAPSULE Metal-organic framework Template 

分 类 号：TQ426.97[化学工程]