HPLC-ELSD法测定3种广式凉果中的返砂糖  

Determination of Granulated Sugar in Three Kinds of Guangdong-Style Preserved Fruits by HPLC-ELSD

作　　者：张增榜 张国列 张泽佳 黄智兴 李锦添 吴巨贤[3,4] 黄百祺[3,4] ZHANG Zengbang;ZHANG Guolie;ZHANG Zejia;HUANG Zhixing;LI Jintian;WU Juxian;HUANG Baiqi(Guangdong Tongxiang Food Co.,Ltd.,Jieyang 515300,Chian;School of Biotechnology,Guangdong Food and Drug Vocational College,Guangzhou 510520,China;School of Catering and Tourism,Guangdong Polytechnic of Science and Trade,Guangzhou 510430,China;Modern Lingnan Food Heritage&Innovation Center of Guangdong Polytechnic of Science and Trade,Guangzhou 510430,China)

机构地区：[1]广东同享食品有限公司,广东揭阳515300 [2]广东食品药品职业学院生物技术学院,广东广州510520 [3]广东科贸职业学院餐旅学院,广东广州510430 [4]广东科贸职业学院现代岭南饮食传承创新研发广东省普通高校工程技术中心,广东广州510430

出　　处：《现代食品》2021年第4期208-211,218,共5页Modern Food

基　　金：广东省普通高校工程技术研究(开发)中心科研项目(编号:2020GCZX015);广东科贸职业学院科研项目(编号:GDKM2019-A09)

摘　　要：本文建立了高效液相色谱-蒸发光散射法检测广式凉果返砂糖中单糖和双糖的方法。采用Shodex Asahipak NH2P-504E(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-水(70∶30,V/V),流速为1 mL·min^(-1),柱温为25℃,蒸发光检测器以N2为载气,压力为350 kPa,漂移管温度为60℃。结果表明,蔗糖、葡萄糖和果糖三者达到基线分离,3种糖在1~10 mg·mL^(-1)的浓度范围内线性关系良好,加标回收率在95.12%~104.65%。3种凉果返砂糖中未检测到蔗糖,主要含有葡萄糖和果糖。This paper established a high performance liquid chromatography-evaporative light scattering method for the detection of monosaccharides and disaccharides in granulated sugar of Guangdong-style preserved fruits.Using Shodex Asahipak NH2P-504E(4.6 mm×250 mm,5μm)chromatographic column,the mobile phase is acetonitrile-water(70∶30,V/V),the flow rate is 1 mL·min^(-1),and the column temperature is 25℃,The evaporative light detector uses N2 as the carrier gas,the pressure is 350 kPa,and the drift tube temperature is 60℃.The results showed that sucrose,glucose and fructose reached the baseline separation,the three sugars had a good linear relationship within the concentration range of 1~10 mg·mL^(-1),and the spiked recovery rate was 95.12%~104.65%.Sucrose is not detected in granulated sugar of three kinds of Guangdong-style preserved fruits,and it mainly contains glucose and fructose.

关 键 词：高效液相色谱-蒸发光散射法 凉果 砂糖 含量测定 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.7[轻工技术与工程—食品科学与工程]