L-丙氨酸转化菌发酵条件的优化  

Fermentation Conditions Optimization of L-Alanine-producing Bacteria

作　　者：徐慧 崔颖 王珊珊[1] 田延军 贺强之 韩延雷 刘建军[1] 朱坤福 祝蕾 姜国政 XU Hui;CUI Ying;WANG Shan-shan;TIAN Yan-jun;HE Qiang-zhi;HAN Yan-lei;LIU Jian-jun;ZHU Kun-fu;ZHU Lei;JIANG Guo-zheng(Shandong Food Ferment Industry Research&Design Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250013,Shandong,China;Shandong Zhushi Pharmaceutical Group Co.,Ltd.,Heze 274300,Shandong,China;Yantai Hengyuan Bioengineering Co.,Ltd.,Yantai 265709,Shandong,China)

机构地区：[1]齐鲁工业大学(山东省科学院)山东省食品发酵工业研究设计院,山东济南250013 [2]山东朱氏药业集团有限公司,山东菏泽274300 [3]烟台恒源生物股份有限公司,山东烟台265709

出　　处：《食品研究与开发》2021年第7期177-182,共6页Food Research and Development

基　　金：山东省重大科技创新工程项目(2019JZZY010341);山东省泰山产业领军人才建设项目(鲁政办字<2019>190号);山东省中央引导地方科技发展资金项目(YDZX20203700003052)。

摘　　要：产L-天冬氨酸-β-脱羧酶(L-aspartate-β-decarboxylase,Asd)菌株以L-天冬氨酸(L-aspartic acid,L-Asp)为底物转化生产L-丙氨酸,通过单因素及正交设计试验对睾丸酮丛毛单胞菌HY-08D培养基组成及发酵条件进行优化。结果表明,最佳培养基组成:富马酸1.0%、L-Asp 1.0%、谷氨酸1.0%、玉米浆干粉0.6%、酵母粉0.8%、氯化钠0.7%、磷酸二氢钾0.15%、硫酸镁0.1%,pH 6.0。产酶菌株HY-08D转化用培养液采用三级扩培,一级接二级使用0.1%低接种量,二级接三级使用10%的接种量,最适通气量为10 L/min。优化后培养液中HY-08D菌体量和酶活较初始提高约1倍,培养周期缩短6 h~8 h。The L-aspartate-β-decarboxylase(Asd)-producing strain transforms L-alanine with L-aspartic acid(L-Asp)as substrate.The single factor and orthogonal experiments were employed to optimize the culture medium and fermentation conditions for Asd production by Comamonas testosteroni HY-08D.The optimal fermentation medium consisted of the following:fumaric acid 1.0%,L-Asp 1.0%,glutamic acid 1.0%,corn starch powder 0.6%,yeast extract 0.8%,NaCl 0.7%,KH2PO40.15%,MgSO40.1%,pH 6.0.The medium for the transformation of HY-08D strain was expanded in three-stage culture.A low inoculation amount of 0.1%was used from the first to the second stage.10%quantity of inoculation was used from the second to the third stage.The optimal ventilatory capacity was 10 L/min.The biomass and enzyme activity of HY-08D in optimized medium had doubled,and the culture period was shortened by 6 h-8 h compared with the initial state.

关 键 词：L-丙氨酸 睾丸酮丛毛单胞菌 发酵条件 酶活 优化 

分 类 号：TQ922.2[轻工技术与工程—发酵工程]