哈蟆油蛋白多肽的透皮作用研究  

Study on the Skin Permeation Effect of Ranae Oviductus Peptides

作　　者：张玉尧 徐云凤 王涵 李香艳[1] 荆礼 张鹤[1,3] Zhang Yuyao;Xu Yunfeng;Wang Han;Li Xiangyan;Jing Li;Zhang He(Changchun University of Chinese Medicine/Jilin Provincial Key Laboratory of BioMacromolecules of Chinese Medicine,Changchun 130117;GenScience Pharmaceuticals Co.,Ltd.,Changchun 130012;Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130021)

机构地区：[1]长春中医药大学/吉林省中药生物大分子重点实验室,长春130117 [2]长春金赛药业有限责任公司,长春130012 [3]长春中医院大学附属医院,长春130021

出　　处：《中国农学通报》2021年第8期33-38,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然基金青年科学基金项目“基于蛋白质组学技术的哈蟆油鉴别和质量评价研究”(81503243);吉林省科技发展计划项目“吉林省中药生物大分子研究与应用创新团队”(20190101010JH);吉林省科技发展计划项目“中药复方化妆品机制研究及系列产品开发”(20190304095YY)。

摘　　要：比较酶解前后哈蟆油蛋白多肽的相对分子质量、黏度和透皮情况。采用胃蛋白酶-碱性蛋白酶双酶法制备哈蟆油蛋白多肽,应用旋转粘度计测定酶解过程中黏度变化;以离体小鼠的皮肤为透皮屏障,应用透皮吸收仪测定不同透皮时间透皮液中蛋白多肽的透皮率和分子量分布。双酶法制备的哈蟆蛋白多肽黏度从5278.85 m Pa·s(1.0 g/100 m L)降到12.56 mPa·s(7.0 g/100 m L),分子质量1147 k Da蛋白质从75.36%降到8.83%,透皮率从4.50%增加到9.14%。透皮36 h时,透皮液中主要为分子质量小于1 kDa哈蟆油蛋白多肽约占62%,其次是分子质量2.46 kDa的蛋白多肽占13.34%。双酶法制备的哈蟆油蛋白多肽分子量低、水溶性好,易于透过皮肤吸收。A comparative study on relative molecular mass, viscosity and skin permeation effect of ranaeoviductus peptides before and after enzymolysis was carried out. Ranae oviductus peptides were preparedthrough hydrolyzing ranae oviductus protein by pepsin and alkaline protease. The viscosimeter was used todetect the changes of viscosity during the enzymatic hydrolysis process. Taking the mouse skin as atransdermal barrier, percutaneous rate and the relative molecular weight distribution of percutaneous peptidesat different times were detected by apparatus of transdermal absorption and high performance liquidchromatograph(HPLC), respectively. The results showed that the viscosity of ranae oviductus peptidesdecreased from 5278.85 m Pa·s(1.0 g/100 m L) to 12.56 m Pa·s(7.0 g/100 m L);the peak area percentage ofranae oviductus proteins with the relative molecular mass 1147 k Da went down from 75.36% to 8.83%,however, the percutaneous rate of ranae oviductus peptides increased from 4.50% to 9.14%. When thepercutaneous time was 36 h, percutaneous peptides with the relative molecular mass less than 1 k Da were themain component in percutaneous fluid, accounting for about 62%, followed by percutaneous peptides with therelative molecular mass of 2.46 kDa, accounting for 13.34%. In general, the ranae oviductus peptides prepared by double-enzyme method have low molecular weight and good water solubility, and could penetrate the skin ofmouse easily.

关 键 词：哈蟆油蛋白多肽 双酶法 透皮作用 分子质量 黏度 

分 类 号：S859.4[农业科学—临床兽医学]