ADAR1 shRNA对人白血病细胞株K562细胞增殖的影响  

Effects of shRNA-ADARI on the proliferation of K562 cell line

作　　者：胡彬[1] 张蓉[1] 刘小五 刘赞[1] 王刚峰 梁英民[1] HU Bin;ZHANG Rong;LIU Xiaowu;LIU Zan;WANG Gangfeng;LIANG Yingmin(Department of Hematology,Xi'an Gaoxin Hospital,Shaanxi Xi'an 710075,China)

机构地区：[1]西安高新医院血液科,陕西西安710075

出　　处：《现代肿瘤医学》2021年第8期1292-1295,共4页Journal of Modern Oncology

基　　金：西安市科技计划项目[编号:201805095YX3SF29(5)]。

摘　　要：目的:探讨ADAR1 shRNA对人白血病细胞株K562细胞增殖的影响。方法:利用实时荧光定量聚合酶联反应(quantitative real time PCR,qRT-PCR)和蛋白质印记法(Western-blot)检测ADAR1在慢性粒细胞白血病(急变期)患者和正常成人中的表达。培养人白血病细胞株K562细胞,用慢病毒介导的siRNA转染K562细胞,建立稳定的敲减ADAR1的K562细胞株(shADAR1),qRT-PCR和Western-blot方法检测K562细胞和shADAR1细胞中ADAR1的mRNA和蛋白的表达。MTT法检测细胞增殖情况。结果:ADAR1的mRNA表达和蛋白水平在慢性粒细胞白血病(急变期)患者中的表达明显高于正常成人(P<0.05)。shADAR1细胞中ADAR1的mRNA和蛋白表达明显低于K562细胞(P<0.05)。shADAR1细胞增殖率明显低于K562细胞(P<0.05)。结论:ADAR1在慢性粒细胞白血病(急变期)患者中表达增加,通过siRNA抑制ADAR1的表达能明显抑制人白血病细胞株K562细胞增殖,ADAR1基因可能成为治疗慢性粒细胞白血病的新靶点。Objective:To investigate the effects of shRNA-ADAR1 on the proliferation of human K562 cell line.Methods:The expression of ADAR1 in patients with chronic myeloid leukemia(BP)and normal adults was detected by real-time fluorescence quantitative polymerase chain reaction and Western-blot.A stable K562 cell line(shADAR1)with knockdown ADAR1 was established by culturing human leukemia cell line K562 cells with lentivirus mediated siRNA.The expression of mRNA and protein of ADAR1 in K562 cells and sh ADAR1 cells was detected by qRT-PCR and Western-blot,and the proliferation of K562 cells was detected by MTT assay.Results:The expression of mRNA and protein level of ADAR1 in patients with chronic myeloid leukemia(BP)was significantly higher than that in normal adults(P<0.05).The expression of mRNA and protein in ADAR1 in shADAR1 cells was significantly lower than that in K562 cells(P<0.05).Down-regulation of ADAR1 could inhibit cell proliferation(P<0.05).Conclusion:The expression of ADAR1 is increased in patients with chronic myeloid leukemia(BP).Inhibition of ADAR1 expression by siRNA can significantly inhibit the proliferation of human leukemia cell line K562.ADAR1 gene may be a new target for the treatment of chronic myeloid leukemia.

关 键 词：ADAR1 慢性粒细胞白血病 K562 

分 类 号：R733.7[医药卫生—肿瘤]