马尾藻岩藻聚糖硫酸酯抑制HepG2细胞的增殖研究  

Inhibition Effect of Fucoidan from Sargassum henslowianum on the Proliferation of HepG2 Cells

作　　者：刘诗颖 何坤燕 李英华 朱冰[3] 谌素华[1,4] 秦小明 钟赛意[1,2] 高加龙 陈建平[1,2] LIU Shiying;HE Kunyan;LI Yinghua;ZHU Bing;CHEN Suhua;QIN Xiaoming;ZHONG Saiyi;GAO Jialong;CHEN Jianping(College of Food Science and Technology,Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing,Guangdong Ocean University,Zhanjiang,Guangdong 524088,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian,Liaoning 116034,China;Center Laboratory,Guangzhou Women and Children's Medical Center,Guangzhou Medical University,Guangzhou,Guangdong 510120,China;Shenzhen Research Institute,Guangdong Ocean University,Shenzhen,Guangdong 518108,China)

机构地区：[1]广东海洋大学食品科技学院,广东省亚热带果蔬加工科技创新中心,广东湛江524088 [2]海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁大连116034 [3]广州医科大学附属广州市妇女儿童医疗中心中心实验室,广东广州510120 [4]广东海洋大学深圳研究院,广东深圳518108

出　　处：《农产品加工》2021年第5期22-25,共4页Farm Products Processing

基　　金：广东海洋大学校级大学生创新创业项目(570119023);广东海洋大学创新强校项目(230419100);广东海洋大学南海学者人才项目(QNXZ201909);湛江市科技计划项目(2020A01034);广东省自然科学基金项目(2020A1515010860)。

摘　　要：采用MTT方法考查不同浓度的马尾藻岩藻聚糖硫酸脂对HepG2细胞存活率的影响,进一步运用流式细胞仪和检测ROS含量探讨马尾藻岩藻聚糖硫酸酯抑制HepG2细胞增殖的机理。结果表明,在0.5~8.0 mg/mL内,HepG2细胞的存活率随着马尾藻岩藻聚糖硫酸脂浓度的升高呈下降趋势。研究发现,随着马尾藻岩藻聚糖硫酸脂浓度的增加,细胞内SubG1和G0/G1的细胞数量升高。而且,通过检测细胞内活性氧(Reactive oxygen species,ROS)含量,发现随着马尾藻岩藻聚糖硫酸脂浓度的增加,细胞内ROS含量显著升高。马尾藻岩藻聚糖硫酸脂抑制HepG2细胞增殖是通过诱导细胞内ROS的过量产生导致细胞发生凋亡和G0/G1阻滞。MTT method was used to investigate the effects of different concentrations of fucoidan from Sargassum henslowianum on the cell viability of HepG2 cells.Further,flow cytometry and ROS content were used to investigate the inhibition mechanism of fucoidan from Sargassum henslowianum on proliferation of HepG2 cells.The results showed that within 0.5~8.0 mg/m L,the cell viability of HepG2 cells gradually decreased with the increasing of fucoidan concentrations.Further studies found that the number of SubG1 and G0/G1 cells were increased with the increase in the concentrations of fucoidan from Sargassum henslowianum.Moreover,with the increasing of fucoidan concentrations,the content of ROS significantly increased by detecting reactive oxygen species(ROS)content.In summary,fucoidan from Sargassum henslowianum inhibited HepG2 cells growth by mediating ROS overproduction to induce cell apoptosis and G0/G1 phase arrest.

关 键 词：马尾藻岩藻聚糖硫酸脂 HEPG2细胞 抑制细胞增殖 

分 类 号：R735[医药卫生—肿瘤]