Effects of undigested protein-rich ingredients on polarised small intestinal organoid monolayers  

作　　者：Soumya K.Kar Bart van der Hee Linda M.P.Loonen Nico Taverne Johanna J.Taverne-Thiele Dirkjan Schokker Mari A.Smits Alfons J.M.Jansman Jerry M.Wells 

机构地区：[1]Host Microbe Interactomics Group,Wageningen University&Research,De Elst 1,6708 WD Wageningen,The Netherlands [2]Laboratory of Microbiology,Wageningen University&Research,Stippeneng 4,6708 WE Wageningen,The Netherlands [3]Wageningen Livestock Research,Wageningen University&Research,De Elst 1,6708 WD Wageningen,The Netherlands

出　　处：《Journal of Animal Science and Biotechnology》2020年第4期1026-1032,共7页畜牧与生物技术杂志（英文版）

基　　金：the financial support from the Wageningen University&Research “IPOP Customized Nutrition” program(grant no.4417801270);financed by Wageningen University&Research,the Dutch Ministry of Economic Affairs, Agriculture&Innovation, the graduate school Wageningen Institute of Animal Science(WIAS);industrial partners Trouw Nutrition, The Netherlands and Darling Ingredient International, The Netherlands;supported by the applied and engineering sciences division of The Netherlands Organisation for Scientific Research(NWO project 14935)and DSM Nutritional Products。

摘　　要：Here, we describe the use of monolayers of intestinal epithelial cells derived from intestinal organoids and transcriptomics to investigate the direct effects of dietary protein sources on epithelial function. Mechanically dissociated 3 D organoids of mouse duodenum were used to generate a polarized epithelium containing all cell types found in the tissue of origin. The organoid-derived cell monolayers were exposed to 4%(w/v) of ‘undigested(non-hydrolysed)-soluble' fraction of protein sources used as feed ingredients [soybean meal(SBM) and casein], or alternative protein sources(spray dried plasma protein, and yellow meal worm), or controls for 6 h prior to RNA isolation and transcriptomics. All protein sources altered expression of unique biological processes in the epithelial cells. Exposure of intestinal organoids to SBM downregulated expression of retinol and retinoid metabolic processes as well as cholesterol and lipid biosynthetic pathways, consistent with the reported hypotriglyceridaemic effect of soy protein in vivo. These findings support the use of intestinal organoids as models to evaluate complex interactions between dietary ingredients and the intestinal epithelium and highlights some unique host effects of alternative protein sources in animal feed and potentially human food.

关 键 词：Alternative protein sources CASEIN Intestinal organoids ORGANOIDS Soybean meal Spray dried plasma protein TRANSCRIPTOMICS Yellow meal worm 

分 类 号：S816[农业科学—饲料科学]