UPLC-Q-Trip-MS/MS法测定赤芍中4种成分的含量  

Determination of Four Components in Red Peony by UPLC-Q-Trip-MS/MS

作　　者：孙辑凯[1] 张梅娟[2] 张宏莲[1] 李宏铃[1] 董巍[1] SUN Ji-kai;ZHANG Mei-juan;ZHANG Hong-lian;LI Hong-ling;DONG Wei(College of Pharmacy,Qiqihar Medical University,Qiqihar 161006,China;Academy of Life Science,Qiqihar University,Qiqihar 161000,China)

机构地区：[1]齐齐哈尔医学院药学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔大学生命科学院,黑龙江齐齐哈尔161000

出　　处：《化学试剂》2021年第2期216-219,共4页Chemical Reagents

基　　金：黑龙江省教育厅基本科研业务费资助项目(2016-KYYWF-0861)。

摘　　要：基于超高效液相色谱-三重四级杆串联质谱(UPLC-Q-Trip-MS/MS)技术,建立赤芍中4种化学成分含量测定方法。采用Agilent ZORBAX SB-C18色谱柱(2.1 mm×100 mm,1.8μm),流动相为甲醇-0.1%甲酸水溶液进行梯度洗脱,柱温40℃,流速0.3 mL/min,进样量5μL;质谱条件为Agilent ultro-triple quad LC/MS在线系统,采用多反应监测模式(MRM)分别在正、负离子模式下对色谱流出物进行检测,喷雾电压3.5 kV,干燥气温度380℃,干燥气流量60 L/h,雾化器压力60 MPa。芍药苷、儿茶素、没食子酸和苯甲酸分别在5~50、0.5~5.0、0.25~2.5、0.25~2.5μg/mL范围内与峰面积呈良好线性关系。7批赤芍中芍药苷、没食子酸、儿茶素和苯甲酸的含量均值依次为2.13%、0.047%、0.20%、7.15%。建立的UPLC-Q-trip-MS/MS方法具有良好的定性和定量分析能力,适用于定量分析赤芍中4种化学成分。A method for the determination of four chemical components in red peony roots was established based on ultra-performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-Q-Trip-MS/MS).Agilent ZORBAX SB-C18 column(2.1 mm×100 mm,1.8μm)was chosen as stationary phase,and methanol-0.1%formic acid aqueous solution was the mobile phase for gradient elution.The flow rate was 0.3 mL/min and the column temperature was 40℃.The injection volume was 5μL.The mass spectrum conditions were that multiple reaction monitoring mode(MRM)mode was set to detect chromatographic effluent in positive and negative ion modes on the Agilent ultivo triple quad LC/MS online system.Spray voltage was set at 3.5 kV,and the drying gas temperature was 380℃with gas flow was 60 L/h.The atomizer pressure was 60 MPa.The linear range of paeoniflorin,catechin,gallic acid and benzoic acid were 5~50,0.5~5.0,0.25~2.5,0.25~2.5μg/mL,respectively.In 7 batches of red peony,the average contents of paeoniflorin,gallic acid,catechin and benzoic acid were 2.13%,0.047%,0.20%,and 7.15%,respectively.The UPLC-Q-trip-MS/MS method had good qualitative and quantitative analysis capabilities,which was suitable for the quantitative analysis of four chemical components in red peony.

关 键 词：赤芍 芍药苷 没食子酸 儿茶素 苯甲酸 定量分析 检测 

分 类 号：R917[医药卫生—药物分析学]