乳铁蛋白对大鼠上颌扩弓及复发过程中腭中缝骨吸收的影响及机制探究  

Study of the mechanism of the effectiveness of lactoferrin on the mid-palatal suture bone resorption during the palatal expansion and relapse process

作　　者：程烨 李天成 陈建伟 邹淑娟 李煌[1] 李贵凤[1] CHENG Ye;LI Tiancheng;CHEN Jianwei;ZOU Shujuan;LI Huang;LI Guifeng(Nanjing Stomatological Hospital,Medical School of Nanjing University,Nanjing 210029)

机构地区：[1]南京大学医学院附属口腔医院•南京市口腔医院,210029 [2]四川大学华西口腔医院

出　　处：《现代口腔医学杂志》2021年第1期5-8,共4页Journal of Modern Stomatology

摘　　要：目的研究乳铁蛋白(lactoferrin,LF)对大鼠上颌扩弓及复发过程中腭中缝破骨活动的影响,并初探其作用机制。方法对大鼠扩弓及复发模型给予剂量为1g/kg/d的LF灌胃,用免疫组织化学染色的方法观察LF对腭中缝破骨活动的影响,检测OPG/RANKL/RANK作用轴关键因子的表达变化。结果与单纯扩弓组相比,LF灌胃组腭中缝区域破骨活动相对减弱,骨保护素(osteoprotegerin,OPG)与核因子KB受体活化因子配体(receptor or activator of nuclear factor-KB ligand,RANKL)表达量的比值显著增加。结论 1g/kg/d LF灌胃可以在大鼠上颌扩弓及复发的过程中抑制腭中缝的破骨活动,此作用可能是通过对OPG/RANKL/RANK作用轴的调控实现的。Objective To evaluate the effects of lactoferrin(LF)on rat bone resorption of the mid-palatal suture during maxillary expansion and relapse,and to preliminarily explore the mechanism.Methods The rat maxillary expansion and relapse model was gavaged daily with LF at the dose of 1 g/kg/d.Immunohistochemical staining was conducted to assess the effects of LF on bone resorption of mid-palatal suture.The expressions of key factors in the osteoprotegerin(OPG)/receptor or activator of nuclear factor-KB ligand(RANKL)/RANK axis were detected.Results Compared to the expansion only group,bone resorption was inhibited in the mid-palatal suture of the LF intervention group,accompanied by significantly increased expression ratio of OPG/RANKL.Conclusion LF gavage at 1 g/kg/d can suppress rat bone resorption of the mid-palatal suture during maxillary expansion and relapse,probably by regulating the OPG/RANKL/RANK axis.

关 键 词：乳铁蛋白 腭中缝 破骨 

分 类 号：R780.2[医药卫生—口腔医学]