透明质酸酶的研究进展  

Research progress of hyaluronidases

作　　者：雷曦 张蕊[1,2,3,4] 黄遵锡[1,2,3,4] 周峻沛 LEI Xi;ZHANG Rui;HUANG Zunxi;ZHOU Junpei(College of Life Science,Yunnan Normal University,Kunming,Yunnan 650500,China;Engineering Research Center of Sustainable Development and Utilization of Biomass Energy,Ministry of Education,Kunming,Yunnan 650500,China;Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment,Kunming,Yunnan 650500,China;Key Laboratory of Yunnan Provincial Education Department for Plateau Characteristic Food Enzymes,Kunming,Yunnan 650500,China)

机构地区：[1]云南师范大学生命科学学院,云南昆明650500 [2]生物能源持续开发利用教育部工程中心,云南昆明650500 [3]云南省生物质能与环境生物技术重点实验室,云南昆明650500 [4]云南省高校高原特色食品酶重点实验室,云南昆明650500

出　　处：《微生物学通报》2021年第3期882-895,共14页Microbiology China

基　　金：国家重点研发计划(2017YFB0308401);云南省基础研究计划重点(202001AS070022);云南省万人计划青年拔尖人才(YNWR-QNBJ-2018-383);云南师范大学联大青年学者培养计划(01200205020516036)。

摘　　要：透明质酸酶是能降解透明质酸及部分糖胺聚糖的一类糖苷酶,可应用于医疗和美容等领域。透明质酸酶也可用于制备小分子糖胺寡糖,许多研究发现小分子糖胺寡糖具有比大分子糖胺聚糖更高的生物免疫活性。为便于研究人员对透明质酸酶进行进一步的基础研究及应用研究,本文介绍了透明质酸和透明质酸酶,梳理了透明质酸酶的分类、结构和催化机理,归纳总结了透明质酸酶的酶活力测定方法、重组表达、酶学性质和应用,展望了透明质酸酶的研究方向。Hyaluronidases,a type of glycosidases,degrade hyaluronan and some glycosaminoglycans.These enzymes have a wide range of promising applications in the medical treatment,cosmetology,preparation of oligo-glycosaminoglycans,and so on.Many studies show that oligo-glycosaminoglycans have a lower molecular weight but a higher biological immune activity than glycosaminoglycans.In view of these important functions and applications of hyaluronidases,this review provides useful information to researchers for further theoretical studies and utilization of these enzymes.This review mainly introduces the definitions of hyaluronan and hyaluronidase,as well as the advances of classification,structures,catalytic mechanisms,enzymatic assay methods,recombinant expression,biochemical properties,and applications of hyaluronidases.This review also indicates future research directions of hyaluronidases.

关 键 词：透明质酸 糖胺聚糖 透明质酸酶 透明质酸寡糖 抗氧化性 

分 类 号：Q936[生物学—微生物学]