屎肠球菌R2发酵豆腐黄浆水的代谢作用  

Fermentation Properties of Enterococcus faecium R2 for Tofu Whey

作　　者：陈志娜 叶韬 张慧宇 郭晓香 马琳琳 李金凤 张萍 沈业桥 刘天 Chen Zhina;Ye Tao;Zhang Huiyu;Guo Xiaoxiang;Ma Linlin;Li Jinfeng;Zhang Ping;Shen Yeqiao;Liu Tian(School of Bioengineering,Huainan Normal University,Huainan 232038,Anhui;Key Laboratory of Bioresource and Environmental Biotechnology of Anhui Higher Education Institutes,Huainan 232038,Anhui)

机构地区：[1]淮南师范学院生物工程学院,安徽淮南232038 [2]资源与环境生物技术安徽普通高校重点实验室,安徽淮南232038

出　　处：《中国食品学报》2021年第3期360-368,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：淮南市科技局科技计划项目(2018A365);安徽省自然科学基金青年项目(1908085QC122)。

摘　　要：为研究屎肠球菌R2对豆腐黄浆水的代谢作用,采用高效液相色谱法测定发酵过程中豆腐黄浆水中的低聚糖、有机酸及大豆异黄酮的组分和含量,对α-半乳糖苷酶和β-葡萄糖苷酶进行初步定位及活性测定,并测定发酵黄浆水对DPPH自由基的清除能力。结果表明,发酵过程中,豆腐黄浆水中的水苏糖、棉籽糖、葡萄糖、蔗糖和果糖均被屎肠球菌R2利用,其中葡萄糖、水苏糖和果糖的利用率最高。发酵36 h后,豆腐黄浆水的pH值降到3.58,有机酸总量显著升高,其中乳酸和醋酸的变化最明显。同时,大豆异黄酮的组分及含量发生显著变化,糖苷型大豆异黄酮转变为游离苷元型大豆异黄酮,大大提高了豆腐黄浆水对DPPH自由基的清除能力。此外,屎肠球菌R2具有α-半乳糖苷酶和β-葡萄糖苷酶活性,且两种酶均为胞内酶,且多数为不可溶性蛋白酶。In order to investigate the fermentation properties of Enterococcus faecium R2 for tofu whey,the contents and constituents of oligosaccharides,organic acids and soybean isoflavones in fermented tofu whey(FTW)were measured by high performance liquid chromatography(HPLC),the activities and locations ofα-galactosidase andβ-glucosidase were detected,and as well as the DPPH radical scavenging activities.The results showed that all the sugars including stachyose,raffinose,glucose,fructose and sucrose in the tofu whey were utilized during the fermentation process,especially for the glucose,stachyose and fructose.The total organic acids increased largely and the pH dropped to 3.58 after 36 h fermentation,especially for the lactic acid and acetic acid.Besides,isoflavone glucoside could be transformed into isoflavone aglucone during the fermentation with Enterococcus faecium R2,and resulted in the significantly(P<0.05)increase of the DPPH radical scavenging activities.In addition,the activities ofα-galactosidase andβ-glucosidase were detected in Enterococcus faecium R2,and both ofα-galactosidase andβ-glucosidase were intracellular,and most of which were insoluble.

关 键 词：屎肠球菌 豆腐黄浆水 代谢 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.72[轻工技术与工程—食品科学与工程]