PCR-DGGE法分析不同酒曲中细菌多样性  

The diversity of bacterial in different distiller’s yeast using the method of PCR-DGGE

作　　者：姚淑敏[1] 刘合凤 高飞[1] 张雅倩 孔雪玉 杨重驿 YAO Shumin;LIU Hefeng;GAO Fei;ZHANG Yaqian;KONG Xueyu;YANG Chongyi(College of Life Science,Qufu Normal University,273165,Qufu,Shandong,PRC)

机构地区：[1]曲阜师范大学生命科学学院,山东省曲阜市273165

出　　处：《曲阜师范大学学报（自然科学版）》2021年第2期89-94,共6页Journal of Qufu Normal University(Natural Science)

基　　金：国家自然科学基金(31200400);曲阜师范大学教学改革项目(jg16027).

摘　　要：目的:研究不同酒曲中细菌的多样性.方法:利用DNA提取试剂盒,分别提取6种酒曲的总DNA,利用细菌16S rRNA gene V3区通用引物,采用变性梯度凝胶电泳(polymerase chain reaction-denatured gradient gelelectrophoresis,PCR-DGGE)方法,进行不同酒曲中细菌多样性的分析.结果:6种酒曲中共分离出12属的细菌,分别为:湖南韶山的酒曲中主要是Weissella,Streptophyta,Bacillus,Pantoea,Okibacterium这5个属的菌株,湖南湘潭酒曲中主要含有Weissella,Pediococcus,Lactococcus,Sphingomonas,Streptophyta,Pantoea这6属的菌株,湖南祁东酒曲中主要含有Weissella,Pediococcus,Bacillus,Klebsiella,Pantoea 5属的菌株,福建福州酒曲含有Weissella,Pediococcus,Pantoea,Pediococcus,Sphingomonas,Streptophyta 6属的菌株,浙江丽水酒曲含有Weissella,Pantoea,Pediococcus,Klebsiella,Enterobacter,Rhizobium,Sphingomonas,Thauera 8属的菌株,浙江兰溪酒曲含有Weissella,Streptophyta,Pantoea 3属的菌株.结论:这说明不同地区的酒曲中的细菌的种类有所不同,同时也说明酒曲中Weissella属的菌是发酵的常见菌群.Purpose:To study the bacterial diversity in different distiller’s yeast.Method:Sample preparation of six kinds of distiller’s yeast,DNA extraction,16S rRNA gene V3 PCR amplification,excision of DGGE bands and sequencing were used in this study.Results:Take advantage of polymerase chain reaction-denaturing gradient gel electrophoresis,the genus of Weissella,Streptophyta,Bacillus,Pantoea,Okibacterium were determinded in distiller’s yeast of Hunan Shaoshan;the genus of Weissella,Pediococcus,Lactococcus,Sphingomonas,Streptophyta,Pantoea were determinded in distiller’s yeast of Hunan Xiangtan;the genus of Weissella,Pediococcus,Bacillus,Klebsiella,Pantoea were determinded in distiller’s yeast of Hunan Qidong;the genus of Weissella,Pediococcus,Pantoea,Pediococcus,Sphingomonas,Streptophyta were determinded in distiller’s yeast of Fujian Fuzhou;the genus of Weissella,Pantoea,Pediococcus,Klebsiella,Enterobacter,Rhizobium,Sphingomonas,Thauera were determinded in distiller’s yeast of Zhejiang Lishui;the genus of Weissella,Streptophyta,Pantoeawere determinded in distiller’s yeast of ZheJiang LanXi;Conclusions:This work shows that the bacteria diversity is abundant in different distiller’s yeast and the genus of Weissella were the greatest group as well.

关 键 词：变性梯度凝胶电泳(PCR-DGGE) 酒曲 16S rRNA gene V3区 细菌多样性 

分 类 号：TS261.1[轻工技术与工程—发酵工程]