葛叶多酚的提取工艺及其抗氧化性质  

Extraction technology of polyphenols from Pueraria lobata leaf and its antioxidant properties

作　　者：唐婷范[1,2] 朱家庆 李荣彬 冯军 黄芳丽[1,2] 梁杰婷 田玉红 李晓慧[1,2] 程昊[1,2] TANG Tingfan;ZHU Jiaqing;LI Rongbin;FENG Jun;HUANG Fangli;LIANG Jieting;TIAN Yuhong;LI Xiaohui;CHENG Hao(School of Biological and Chemical Engineering,Guangxi University of Science and Technoloy,Liuzhou 545006,China;Guangxi Key Laboratory of Green Processing of Sugar Resources(Guangxi University of Science and Technoloy),Liuzhou 545006,China)

机构地区：[1]广西科技大学生物与化学工程学院,广西柳州545006 [2]广西糖资源绿色加工重点实验室(广西科技大学),广西柳州545006

出　　处：《广西科技大学学报》2021年第2期100-106,共7页Journal of Guangxi University of Science and Technology

基　　金：广西研究生教育创新计划项目(GKYC202012);国家自然科学基金地区科学基金项目(21662003);广西糖资源绿色加工重点实验室主任基金(KY2016YB250);大学生创新训练计划项目(202010594214);广西科技大学博士基金项目(校科博14Z06).

摘　　要：采用回流法提取葛叶中的多酚,用福林酚法测定葛叶多酚含量,通过精密度、稳定性和加标回收率试验验证适用性,对葛叶多酚的抗氧化性质进行研究,并利用单因素实验研究对葛叶多酚得率的影响.结果显示葛叶多酚最佳提取工艺为:溶剂pH值为4、料液比1∶30、70℃水浴2.0 h,提取2次,精密度实验相对标准误差为0.27%,稳定性实验相对标准误差为0.94%,加标回收率实验相对标准误差为1.66%,此条件下葛叶多酚得率为2.22%;葛叶多酚对DPPH·和·OH具有较强的清除作用,最大清除率分别为80.99%和76.24%.回流法提取葛叶多酚工艺合理,操作简单,且提取后的葛叶多酚有较强的抗氧化性,为葛叶多酚的开发利用提供基础性研究.The polyphenols in Pueraria lobata were extracted by reflux method,and the content of polyphenols in Pueraria lobata was determined by Folin phenol method.The applicability of polyphenols in Pueraria lobata was verified by precision,stability and standard addition recovery tests and the antioxidant properties of polyphenols from Pueraria lobata were studied.The single factor experiment was used to study the effect on the yield of polyphenols from Pueraria lobata.The results showed that the optimum extraction conditions were as follows:solvent pH=4,solid-liquid ratio 1∶30,70℃water bath for 2.0 h,the relative standard error of precision test was 0.27%;the relative standard error of stability test was 0.94%;the relative standard error of recovery experiment was 1.66%;under these conditions,the yield of Pueraria leaf polyphenols was 2.22%.Polyphenols from Pueraria lobata had strong scavenging effect on DPPH·and·OH,with the maximum scavenging rates of 80.99%and 76.24%,respectively.The process of extracting polyphenols from Pueraria lobata by reflux method is reasonable and simple,and the extracted polyphenols have strong antioxidant activity,providing basic research for the development and utilization of Pueraria lobata polyphenols.

关 键 词：葛叶 多酚 提取工艺 抗氧化性 

分 类 号：TQ91[化学工程]