玉米赤霉烯酮内酯水解酶的鉴定、改造及应用  

Zearalenone-detoxifying lactonase:Identification,modification and application

作　　者：张振霞 徐炜 吴昊 张文立 光翠娥[1] 沐万孟[1] ZHANG Zhenxia;XU Wei;WU Hao;ZHANG Wenli;GUANG Cui’e;MU Wanmeng(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]食品科学与技术国家重点实验室(江南大学),江苏无锡214122

出　　处：《食品与发酵工业》2021年第7期285-291,共7页Food and Fermentation Industries

基　　金：十三五国家重点研发计划项目(2019YFC1604602);江苏省研究生科研与实践创新计划项目(SJCX20_0742)。

摘　　要：玉米赤霉烯酮(zearalenone,ZEN)及其衍生物是世界范围内污染最严重的一种真菌毒素,对人类生命和财产安全构成了极其严重的威胁。传统的物理和化学脱毒方式因存在脱毒不彻底、破坏食品质构以及导致营养成分流失等弊端而无法满足实际生产应用。近年来,生物酶法降解真菌毒素因微生物来源广泛、酶反应条件温和以及脱毒效率高等诸多优势而逐渐被研究和报道。特别地,玉米赤酶烯酮内酯水解酶(lactonase)因具有较高的ZEN降解活力、明确的脱毒机理、不可逆的脱毒过程和阐明的晶体结构等优点而被广泛研究。文章对微生物来源的内酯水解酶的基因挖掘、性质鉴定、分子改造以及应用探索方面进行了综述。旨在阐明目前内酯水解酶在玉米赤霉烯酮脱毒工业应用中存在的优势与挑战,并为其未来在玉米赤霉烯酮生物降解方面的研究提供参考。Zearalenone and its derivatives are the most widely-existed mycotoxins around the world,causing huge safety hazards and economic losses to humans.Traditional physical and chemical detoxification methods cannot meet the demands of industrial applications due to their incomplete detoxification,destruction to food texture,and loss of nutrients.In recent years,biological and enzymatic degradation of mycotoxins has gradually been studied and reported due to its wide range of microbial sources,mild enzyme reaction conditions and high detoxification efficiency.Particularly,the lactone has been extensively studied due to its high ZEN degradation activity,clear detoxification mechanism,irreversible detoxification process and elucidated crystal structure.This article reviewed the research progress of lactone in terms of gene mining,characterization,molecular modification and application exploration.The purpose is to clarify the advantages and challenges in the industrial application of the zearalenone-detoxifying lactone,and provide certain references for its future research on zearalenone biodegradation.

关 键 词：玉米赤霉烯酮 内酯水解酶 性质鉴定 分子改造 应用 

分 类 号：TS201.2[轻工技术与工程—食品科学]