Origanum vulgare L.leaf extract alleviates finasteride-induced oxidative stress in mouse liver and kidney  

作　　者：Qi-Feng Sun Shi-Xiang Chen Zhang-Feng Tang Xiao-Yi Song Fa Jing Hao-Tian Wu Zhong-Yang Ding Attalla El-kott Diaa Massoud Heba Khalifa 

机构地区：[1]Department of Hepatobiliary and Vascular Surgery,Jinan City People's Hospital,Jinan,Shandong Province,271100,China [2]Department of Hepatology,Zhejiang University Ming Zhou Hospital,Ningbo,Zhejiang Province,315199,China [3]Department of General Surgery,Wuxi Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Traditional Chinese Medicine,Wuxi,Jiangsu Province,214023,China [4]Biology Department,College of Science,King Khalid University,Abha,Saudi Arabia [5]Zoology Department,College of Science,Damanhour University,Damanhour,Egypt [6]Biology Department,College of Science,Jouf University,P.O.Box 2014,Sakaka,Al-Jouf,Saudi Arabia [7]Zoology Department,Faculty of Science,Fayoum University,Fayoum,Egypt

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2021年第5期194-204,共11页亚太热带生物医学杂志（英文版）

基　　金：supported by King Khalid University,Abha,KSA through Research Group Project under grant number R.G.P.2/80/41 and Wuxi Municipal Health and Family Planning Commission(WXQ201832).

摘　　要：Objective:To investigate the hepatorenoprotective effects of Origanum vulgare L.against finasteride-induced oxidative injury in the liver and kidney of mice.Methods:Liquid chromatography-electrospray ionization-tandem mass spectrometry(LC-ESI/MS)analysis was utilized to yield a fingerprint of Origanum vulgare polyphenolic constituents.Thirty BALB/c mice received 0.5 mL/day distilled water,finasteride(25 mg/kg/day for 10 d),and 100,200,or 400 mg/kg/day finasteride+Origanum vulgare extract with 6 mice per group for five weeks.On day 36,liver and kidney function as well as pro-and antiinflammatory(IFN-γ,IL-12,IL-6,TNF-α,IL-1β,and IL-10)cytokines were measured.The total antioxidant status,nitric oxide(NO),and malondialdehyde levels as well as the activities of NO synthase and catalase were also evaluated.Histopathological study was conducted to assess the effect of Origanum vulgare extract on finasteride-induced renal and hepatic toxicities.Results:Twenty-five major polyphenolic compounds were identified in the Origanum vulgare extract by LC-ESI/MS.Origanum vulgare extract,especially at 200 and 400 mg/kg/day doses,significantly improved liver and kidney biochemical indices,decreased inflammatory cytokines,increased total antioxidant status and NO synthase and catalase activities,as well as decreased plasma NO and malondialdehyde levels in a dose-dependent manner as compared to the finasteride group.Histopathological results further confirmed the protective effect of Origanum vulgare extract.Conclusions:Origanum vulgare extract ameliorates finasterideinduced hepatic and renal biochemical and histopathological alterations,and restores antioxidant/oxidant balance.

关 键 词：KIDNEY LIVER Origanum vulgare L. INFLAMMATION ANTIOXIDANT 

分 类 号：R285.5[医药卫生—中药学]