一株红树林来源稀有放线菌的鉴定和抑菌活性物质的初步研究  

Identification and antimicrobial activity of the rare Actinomyces sp.from mangrove sediment

作　　者：张骏梁 张诗玲 吴佳慧 许姗姗 梁锦有 徐颖 ZHANG Junliang;ZHANG Shiling;WU Jiahui;XU Shanshan;LIANG Jinyou;XU Ying(Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science,Shenzhen Engineering Laboratory for Marine Algal Biotechnology,College of Life Sciences and Oceanography,Shenzhen University,Shenzhen 518060,China;Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,China;College of Engineering,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]深圳大学生命与海洋科学学院,深圳市海洋生物资源与生态环境重点实验室,深圳市海洋藻类生物开发与应用工程实验室,广东深圳518060 [2]深圳大学物理与光电工程学院,光电子器件与系统(教育部/广东省)重点实验室,广东深圳518060 [3]中国药科大学工学院,江苏南京211198

出　　处：《食品与发酵工业》2021年第8期101-107,共7页Food and Fermentation Industries

基　　金：深圳市海外高层次人才创新创业专项资金(KQJSCX20170727101743831)。

摘　　要：对分离自香港米埔红树林放线菌XY-R10进行鉴定并研究其抑菌活性。采用R2A和ISP-2培养基分离和纯化菌株。通过PCR扩增和测序获得菌株16S rRNA序列,利用邻接法构建系统发育树并结合形态学分析,初步鉴定该菌可能为拟孢囊菌属的Kibdelosporangium phytohabitans。通过纸片扩散法和微量稀释法研究发酵粗提物的抑菌活性,发现其具有广谱抗革兰氏阳性菌作用。初步分离发现粗提物中可能存在多个活性成分,结合液相-串联高分辨质谱分析,鉴定出活性成分Maipomycin A,它对表皮葡萄球菌、单增李斯特菌和副溶血弧菌有抑菌活性。该文为挖掘红树林微生物资源、抗菌药物的开发,以及食品工业寻找新的抑菌防腐物质提供了研究基础。In this study,we reported the identification and antimicrobial activity of the rare actinomycete XY-R10 collected from the sediment of mangrove in Mai Po wetlands,Hong Kong.Actinobacteria were isolated on R2A plate and purified in ISP-2 medium.The 16S rRNA gene sequences were obtained by PCR amplification and sequencing.Through phylogenetic tree and morphology analysis,it was preliminarily identified that the strain may be Kibdelosporangium phytohabitans KLBMP 1111 T.The antibacterial activity of fermentation extracts was assayed by the disk susceptibility test and micro-dilution test.The extracts showed a broad-spectrum anti-Gram-positive bacteria effect.Preliminary separation found that there were multiple active ingredients in the crude extract.Maipomycin A was identified in the crude extracts by liquid chromatography-tandem high-resolution mass spectrometry(LC-HRMS),and it showed antibacterial activity against Staphylococcus epidermidis,Listeria monocytogenes and Vibrio parahemolyticus.These findings provide a research basis for the exploitation of mangrove microbial resources to discover new drug leads for pharmaceutical industry as well as new antibacterial or antiseptic substances for the food industry.

关 键 词：红树林微生物 稀有放线菌 拟孢囊菌属 抑菌活性 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]