气升式反应器中通气量和pH对双孢蘑菇液体菌种的影响  

Effects of Aeration Volume and pH on Liquid Seeds of Agaricus bisporus in 5L Air-lift Reactor

作　　者：尹川 杜冰冰[2] 连洁 张弘扬 黄亮 班立桐 王玉 YIN Chuan;DU Bing-bing;LIAN Jie;ZHANG Hong-yang;HUANG Liang;BAN Li-tong;WANG Yu(College of Agronomy&Resources and Environment,Tianjin Agricultural University,Tianjin 300384,China;Weifang Vocational College,Weifang Shandong 261041,China;Tianjin Binheidesheng Agricultural Technology Co.,Ltd.,Tianjin 300140,China)

机构地区：[1]天津农学院农学与资源环境学院,天津300384 [2]潍坊职业学院,山东潍坊261041 [3]天津滨海德盛农业科技有限公司,天津300140

出　　处：《食品与发酵科技》2021年第2期35-39,共5页Food and Fermentation Science & Technology

基　　金：天津市种业科技重大专项(16ZXZYNC00210);中央引导地方科技发展专项(20ZYCGSN00380);国家级大学生创新训练项目(201710061040);天津市蔬菜产业体系(ITTVRS2017015);天津市特支计划-青年拔尖人才项目(TJTZJHQNBJRC-2-12)。

摘　　要：为了实现双孢蘑菇液体菌种的规模化应用,以菌丝球生物量、直径、密度、萌发时间等为关键指标,采用5L气升式反应器,对发酵过程通气量和pH进行探索。结果表明:在通气量3.0L/min,控制发酵过程恒定为pH 6.0的条件下,双孢蘑菇液体菌种生物量达到91 g/L,菌丝萌发时间低至6.8 h,密度为67个/mL。本研究为双孢蘑菇液体菌种规模化应用提供了有力的支撑。In order to realize the application of liquid seed of Agaricus bisporus in the cultivation of edible fungi,the growth characteristics of the A.bisporus in 5 L air-lift reactor was investigated.Mycelium biomass,diameter,density and germination time were optimized through control of aeration volume and pH.The results showed that under the condition of aeration volume 3.0 L/min and pH 6.0 mycelium biomass,germination time and density could reached to 91 g/L,6.8 h and 67 per mL,respectively.The study provided a strong support for the application of the A.bisporus liquid seed in the cultivation of mushroom.

关 键 词：双孢蘑菇 液体菌种 气升式反应器 通气量 PH控制 

分 类 号：S646.11[农业科学—蔬菜学]