9株临床结核分枝杆菌全基因组突变情况分析  

Analysis of mutations in the genome of 69 clinical strains of Mycobacterium tuberculosis

作　　者：卓志鹏[1] 蒙粮广 吴清芳[1] 谭卫国[1] ZHUO Zhi-peng;MENG Liang-guang;WU Qing-fang;TAN Wei-guo(Shenzhen Center for Chronic Disease Control,Shenzhen,Guangdong 518020,China)

机构地区：[1]深圳市慢性病防治中心,广东深圳518020

出　　处：《热带医学杂志》2021年第2期171-174,共4页Journal of Tropical Medicine

基　　金：深圳医疗卫生“三名工程”(SZSM201611030)。

摘　　要：目的了解深圳地区初诊结核病人的基因突变情况。方法收集深圳市龙华区慢性病防治中心临床收治的69例结核病人的初诊临床分离菌株,通过二代测序分析比对H37rv菌株分析基因突变情况。结果序列结果全面且方便应用,每株菌株的基因序列均得以展示和保存。耐药基因突变广泛存在,69株临床菌株中链霉素耐药相关基因rspL(11个)、异烟肼耐药相关基因katG(10个)、利福平耐药相关基因rpoB(9个)的突变广泛存在。利福平基因耐药情况与表型药敏结果具有高度一致性。结论应用全基因组测序技术分析基因突变情况,针对突变结果调整用药或用药剂量,对临床病人的精准治疗可能产生更好的效果。Objective Whole genome sequencing(WGS)were used to analysis the gene mutation characteristics of the first-visit patients’Mycobacterium tuberculosis in Shenzhen.Methods 69 First-visit patients’isolated strains of Mycobacterium tuberculosis in Longhua district of Shenzhen were collected.The result of gene mutations were analyzed by comparing the gene sequence of H37Rv with those of whole genome sequencing of 69 strains.Results WGS results of each patitent were comprehensive and ready for analysis.Drug resistance gene mutations were widespread;the mutations of rspL(11),katG(10)and rpoB(9)in 69 clinical strains were widespread.WGS gene analysis and drug resistance result of rifampin was highly consistent.Conclusion The application of whole-genome sequencing technology to analyze gene mutations and adjust the medication or dosage according to the mutation results could provide precise treatment for patients.

关 键 词：结核分枝杆菌 耐多药结核病 全基因组测序 基因突变 

分 类 号：R378.911[医药卫生—病原生物学]