Protective effect of Fructus Mume total flavone against SH-SY5Y cells damage induced by MPP+and its mechanism  

作　　者：Chun-Ling Wang Xiao-Dong Wen Ning Luo Yuan-Jing Jiang Ying Jiang Zhen Zeng 

机构地区：[1]Guangxi University of Traditional Chinese Medicine,Nanning 530200,China [2]Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning 530011,China

出　　处：《Journal of Hainan Medical University》2021年第7期11-15,共5页海南医学院学报（英文版）

基　　金：National Natural Science Foundation of China Project(No.82060888);Approved Project of Guangxi Health Department(No.Z2016220);Approved Project of Guangxi Administration of Traditional Chinese Medicine(No.GZZC2020107)。

摘　　要：Objective:To investigate the neuroprotective effects of Fructus Mume total flavone(FMF)against cell apoptosis and mitochondrial injury induced by 1-methyl-4-phenylpyridinium(MPP^(+))in human neuroblastoma(SH-SY5Y)cells and explore its molecular mechanisms.Methods:MPP^(+) induced SH-SY5Y cells injury model were established in vitro cell culture,the cells were divided into 5 groups:normal control group,model group(250μmol·L^(-1) MPP^(+)),FMF low-and middle-and high-dose experimental group(10,50,100μmol·L^(-1) FMF).After 72 h administration,4’,6-diamidino-2-phenylindole(DAPI)staining was used to observe the effects of different concentrations of FMF on the morphologic changes of apoptotic cells,the ratio of cell apoptosis was measured by Annexin-FITC/PI double staining.The mitochondrial membrane electro-bit were detected by flow cytometry(FCM).The expression of Bcl-2,Bax and Caspase-3 were detected by Western blot.Results:The results of DAPI staining showed that the injury SH-SY5Y cells induced by MPP+were densely condensed,the nucleus showed nuclear shrinkage,showing an apoptotic characteristic morphology;after 72h of FMF action,the apoptotic morphology of the cells showed different degrees of improvement,and the apoptotic number of SH-SY5Y cells also decreased.Compared with that in the normal control group,the apoptotic rate and of mitochondrial membrane electrobit of SH-SY5Y cells in the model group increased significantly(P<0.01),the expression of Bax and Caspase-3 proteins increased significantly(P<0.01),Bcl-2 protein and the ratio of Bcl-2/Bax decreased significantly(P<0.01).Compared with that in the model group,the apoptotic rate and mitochondrial membrane electro-bit of SH-SY5Y cells in FMF groups(10,50,100μmol·L^(-1))were significantly lower,while Bax and Caspase-3 proteins were significantly lower(P<0.01),and Bcl-2 protein and the ratio of Bcl-2/Bax were significantly higher,with statistically significant difference in FMF middle-and high-dose experimental groups(P<0.01).The results indicated that

关 键 词：Parkinson’s disease Fructus Mume total flavone 1-METHYL-4-PHENYLPYRIDINIUM SH-SY5Y cell Cell apoptosis 

分 类 号：R73[医药卫生—肿瘤]