微生物法生产丙酮酸的代谢工程研究进展  

Research Progress on Metabolic Engineering for Microbial Production of Pyruvate

作　　者：刘云凤 尹良鸿 钱永常[1] LIU Yun-feng;YIN Liang-hong;QIAN Yong-chang(School of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,China)

机构地区：[1]浙江农林大学林业与生物技术学院,浙江杭州311300

出　　处：《药物生物技术》2021年第1期105-110,共6页Pharmaceutical Biotechnology

基　　金：国家自然科学基金(No.31600070);浙江农林大学个人科研发展基金(No.2015FR015)。

摘　　要：丙酮酸是细胞代谢的关键中间产物,为多种氨基酸、油脂、生物碱类、萜类的合成前体,现已广泛应用于食品、医药、化工等领域,市场需求日益增加。丙酮酸可以通过化学合成法、生物催化转化法及微生物发酵法生产。其中微生物发酵法具有原料廉价易得、环境污染小、产物纯度高等优点。文章系统分析了微生物中丙酮酸的生物合成途径和代谢调控,综述了代谢工程在丙酮酸合成中的改造策略及效果,并展望了丙酮酸育种的未来发展趋势。Pyruvate is the key intermediate product of cell metabolism,which is a synthetic precursor of various amino acids,oils,alkaloids and terpenes. It has been widely used in food,medicine,chemical industry and other fields,and the market demand is increasing. Pyruvate is produced by chemical synthesis,biocatalytic conversion and microbial fermentation. Among them,microbial fermentation has the advantages of cheap raw materials,little pollution and high purity of products. The biosynthesis pathway and metabolic regulation of pyruvate in microorganisms are systematically analyzed,the transformation strategy and effect of metabolic engineering in pyruvate synthesis are summarized in this review,the future development trend of pyruvate breeding is prospected.

关 键 词：丙酮酸 代谢工程 糖酵解途径 调节机制 微生物发酵 

分 类 号：TQ92[轻工技术与工程—发酵工程]