微生物发酵法制备牛樟叶粗多糖研究  

Extraction of Crude Polysaccharides from Cinnamomum Kanehirae Leaves by Microbial Fermentation

作　　者：张蓓 余潇苓[2] 万永艳 张洪平 梁学政 ZHANG Bei;YU Xiaoling;WAN Yongyan;ZHANG Hongping;LIANG Xuezheng(Liuzhou Traditional Chinese Medical Hospital,Liuzhou,Guangxi,China 545026;The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,Zhejiang,China 310000)

机构地区：[1]广西壮族自治区柳州市中医医院,广西柳州545026 [2]浙江中医药大学附属第一医院,浙江杭州310000

出　　处：《中国药业》2021年第9期27-30,共4页China Pharmaceuticals

基　　金：国家自然科学基金[81460549];新疆维吾尔自治区自然科学基金[2017D01C169];浙江省基础公益研究计划项目[LQ19H290002];柳州市柳北科学技术局科技攻关与新产品试制项目[LB201801];柳州市阳和工业新区应用技术研究开发与科技创新项目[2019007]。

摘　　要：目的优化牛樟叶粗多糖的微生物发酵工艺。方法以粗多糖得率为指标,以菌种、发酵时间、发酵温度、接菌量为考察因素,在单因素试验基础上采用Box-Benhnken中心组合试验设计优化牛樟叶粗多糖的发酵工艺条件。结果发酵的最佳工艺条件为发酵时间2.23 d,发酵温度25℃,接菌量6%。结论该优化工艺稳定可靠,试验值与建立的模型预测值基本一致。Objective To optimize the microbial fermentation conditions of the extraction of crude polysaccharides from Cinnamomum kanehirae Leaves.Methods With taking the yield of crude polysaccharide as the index,the bacteria strain,fermentation time,fermentation temperature and the inoculation amount of bacteria as the investigation factors,the fermentation conditions of crude polysaccharides from Cinnamomum kanehirae Leaves were optimized by Box-Behnken central composite design based on the single factor test.Results The optimum fermentation conditions were as follows:the fermentation time was 2.23 d,the fermentation temperature was 25℃,and the inoculation amount was 6%.Conclusion The optimized process is stable and reliable,and the experimental value is consistent with the predicted value of the established model.

关 键 词：微生物 发酵法 粗多糖 Box-Benhnken中心组合试验 单因素试验 工艺优化 

分 类 号：R932[医药卫生—生药学] R943[医药卫生—药学]