Isolation of Pleurotus fl orida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice  

作　　者：Kudrat Randhawa Varinder Singh Sanimardeep Kaur Ravinder Kaur Suresh Kumar Richa Shri 

机构地区：[1]Department of Pharmaceutical Sciences and Drug Research,Punjabi University,Patiala 147002,India [2]Chitkara College of Pharmacy,Chitkara University,Punjab,India

出　　处：《Food Science and Human Wellness》2021年第4期490-496,共7页食品科学与人类健康（英文）

基　　金：The study was funded by University Grants Commission under UGC BSR fellowship scheme(Award no.F.4.25-1/2013-14(BSR)/7-265/2009(BSR));The authors are thankful to Prof.Nirmal Singh and Mr.Pankaj Bhatia,Department of Pharmaceutical Sciences and Drug Research,Punjabi University,Patiala,India for their help in carrying out animal studies.The authors are also thankful to DBTIPLS project(Grant no:BT/PR4548/INF/22/146/2012),Punjabi University,Patiala,Punjab,India for providing HPTLC facility and Department of Pharmaceutical Sciences and Drug Research,Punjabi University,Patiala,India for providing the necessary laboratory facilities for this work.

摘　　要：Our earlier work showed that hydromethanol extract(HME)of Pleurotus fl orida had antioxidant and anticholinesterase potential.This study aimed at isolating the constituent responsible for the activities.HME was subjected to bioactivity guided fractionation using in vitro anti-acetylcholinesterase(Ellman method)and antioxidant(DPPH scavenging assay).The most active constituent was evaluated in vivo employing i.c.v.streptozotocin(STZ)induced dementia in mice.Morris water maze test was used for evaluating memory,followed by biochemical estimations and histopathological studies.Bioactivity guided fractionation resulted in isolation of resveratrol(identifi ed by IR,NMR and MS)and its content in P.fl orida fruiting bodies was 0.0098%(m/m,by a validated TLC densitometric method).It improved STZ induced dementia and neurodegeneration in mice by reducing brain acetylcholinesterase action and oxidative stress.The observed effects might be the presence of resveratrol.Our results further endorse that P.fl orida derived resveratrol can be a promising therapy for Alzheimer’s disease.

关 键 词：Acetylcholinesterase inhibitor Alzheimer’s disease MUSHROOMS Pleurotus fl orida RESVERATROL 

分 类 号：TQ464.8[化学工程—制药化工]