陈皮挥发油组成分析及其单体的抗氧化性研究  

Chemical composition of volatile oil from Citri retriculatae pericarpium and its antioxidant activity analysis

作　　者：余祥英 陈晓纯 李玉婷 李琳[1] YU Xiangying;CHEN Xiaochun;LI Yuting;LI Lin(Engineering Research Center of Health Food Design&Nutrition Regulation,School of Chemical Engineering and Energy Technology,Dongguan University of Technology,Dongguan 523808,China)

机构地区：[1]东莞理工学院食品营养健康工程与智能化加工研究中心化学工程与能源技术学院,广东东莞523808

出　　处：《食品与发酵工业》2021年第9期245-252,共8页Food and Fermentation Industries

基　　金：2019年国家农产品质量安全风险评估项目(GJFP2019015);东莞理工学院引进人才科研启动专项经费项目(GC300502-36,GC300502-35);东莞理工学院高层次人才(创新团队)科研启动项目(KCYCXPT2017007)。

摘　　要：为探究陈皮挥发油中抗氧化性的关键组分,采用水蒸气蒸馏法提取新会茶枝柑和福建芦柑陈皮挥发油,利用GC-MS法分析陈皮挥发油组成,以1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)、2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸[2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS]自由基清除能力和还原能力评价陈皮挥发油及其单体的抗氧化性。2种陈皮挥发油中共鉴定出23种成分,均以D-柠檬烯含量最高,γ-萜品烯含量次之,其中新会陈皮挥发油具有相对较低的D-柠檬烯/γ-萜品烯值和倍半萜烯含量,同时邻甲氨基苯甲酸甲酯和香芹酚仅在新会陈皮挥发油中检出。抗氧化性测定结果显示,新会茶枝柑陈皮挥发油相比福建芦柑陈皮挥发油具有基本相同的自由基清除能力和较强的还原能力;单体中香芹酚的抗氧化性最高,弱低于阳性对照V C,单萜烯(萜品油烯、γ-萜品烯和α-松油烯)抗氧化性其次,D-柠檬烯与醛醇类含氧化合物的抗氧化性较低,邻甲氨基苯甲酸甲酯无显著的DPPH自由基清除活性,但具有较强的ABTS自由清除活性和还原能力。结合陈皮挥发油组成和抗氧化性测试表明香芹酚、单萜烯(萜品油烯、γ-萜品烯和α-松油烯)和邻甲氨基苯甲酸甲酯对陈皮挥发油的抗氧化性贡献较大。To explore the potential antioxidants contribution to the antioxidant activity of Citri retriculatae pericarpium volatile oil,the volatile oil was extracted from Xinhui“Chachi”and Fujian“Ponkan”by steam distillation and then was analyzed by GC-MS.In addition,DPPH/ABTS radical scavenging activity and ferric reducing/antioxidant activity were used to evaluate the antioxidant activity of volatile oil and the compounds in volatile oil.The results showed that a total of 23 compounds were identified in Citri retriculatae pericarpium volatile oil from both Xinhui and Fujian,with D-limonene of the highest content andγ-terpinene of the second-highest content.Citri retriculatae pericarpium volatile oil from Xinhui had a lower ratio of D-limonene/γ-terpinene and sesquiterpene.Additionally,methyl o-methylaminobenzoate and carveol could be detected only in Citri retriculatae pericarpium volatile oil from Xinhui.Furthermore,the antioxidant activity test showed that,compared with Citri retriculatae pericarpium volatile oil from Fujian,Citri retriculatae pericarpium volatile oil from Xinhui possesses the same radical scavenging activity and stronger reducing power.Among all tested compounds,carveol possesses the highest antioxidant activity which slightly smaller than Vc,followed by monoterpene(γ-terpinene,terpinolene,α-terpinene).D-limonene,aldehydes and alcohols have a lower antioxidant activity among all tested compounds.However,methyl ortho-methylaminobenzoate had no significant DPPH radical scavenging activity even though it had relatively strong ABTS scavenging activity and reducing ability.Through the composition analysis and antioxidant activity test,carveol,monoterpene(γ-terpinene,terpinolene,α-terpinene)and methyl ortho-methylaminobenzoate could be considered to be the main contributors to the antioxidant activity of volatile oil from Citri retriculatae pericarpium.

关 键 词：陈皮 挥发油 单萜烯 香芹酚 邻甲氨基苯甲酸甲酯 抗氧化性 

分 类 号：TS207.3[轻工技术与工程—食品科学]