Genome-wide distribution and functions of the AAE complex in epigenetic regulation in Arabidopsis  

作　　者：Yi-Zhe Zhang Juncheng Lin Zhizhong Ren Chun-Xiang Chen Daisuke Miki Si-Si Xie Jian Zhang Ya-Nan Chang Jing Jiang Jun Yan Qingshun Q.Li Jian-Kang Zhu Cheng-Guo Duan 

机构地区：[1]Shanghai Center for Plant Stress Biology and CAS Center of Excellence for Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 201602,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems,College of the Environment and Ecology,Xiamen University,Xiamen 361102,China [4]State Key Laboratory of Crop Stress Adaptation and Improvement,School of Life Sciences,Henan University,Kaifeng 475004,China [5]School of Life Sciences,East China Normal University,Shanghai 200241,China [6]Graduate College of Biomedical Sciences,Western University of Health Sciences,Pomona 91766,USA [7]Department of Horticulture and Landscape Architecture,Purdue University,West Lafayette 47907,USA

出　　处：《Journal of Integrative Plant Biology》2021年第4期707-722,共16页植物学报（英文版）

基　　金：supported by the Chinese Academy of Sciences,including the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB27040203 to C.-G.D.and XDB27040000 to J.K.Z);in part by a grant from the National Key Research and Development Project of China(2016YFE0108800 to Q.Q.L.)。

摘　　要：Heterochromatin is widespread in eukaryotic genomes and has diverse impacts depending on its genomic context.Previous studies have shown that a protein complex,the ASI1-AIPP1-EDM2(AAE)complex,participates in polyadenylation regulation of several intronic heterochromatin-containing genes.However,the genome-wide functions of AAE are still unknown.Here,we show that the ASI1 and EDM2 mostly target the common genomic regions on a genome-wide level and preferentially interacts with genetic heterochromatin.Polyadenylation(poly(A)sequencing reveals that AAE complex has a substantial influence on poly(A)site usage of heterochromatin-containing genes,includingnotonlyintronicheterochromatincontaining genes but also the genes showing overlap with heterochromatin.Intriguingly,AAE is also involved in the alternative splicing regulation of a number of heterochromatin-overlapping genes,such as the disease resistance gene RPP4.We provided evidence that genic heterochromatin is indispensable for the recruitment of AAE in polyadenylation and splicing regulation.In addition to conferring RNA processing regulation at genic heterochromatin-containing genes,AAE also targets some transposable elements(TEs)outside of genes(including TEs sandwiched by genes and island TEs)for epigenetic silencing.Our results reveal new functions of AAE in RNA processing and epigenetic silencing,and thus representimportantadvancesinepigenetic regulation.

关 键 词：alternative polyadenylation alternative splicing epigenetic regulation HETEROCHROMATIN TRANSCRIPTION 

分 类 号：Q943.2[生物学—植物学]