响应面分析法优化美国黑莓花青素提取工艺  

Optimization of Extraction Process of Anthocyanins from American Blackberry by Response Surface Methodology

作　　者：吴哲 刘冠群 张娇 冯夏珍 叶松华 阮文辉 WU Zhe;LIU Guanqun;ZHANG Jiao;FENG Xiazhen;YE Songhua;RUAN Wenhui(Center of Drug Inspection for Shanxi,Taiyuan 030031;Institution of Medicine and Life Sciences,Taiyuan 030016)

机构地区：[1]山西省药品检查中心,太原1130031 [2]山西省医药与生命科学研究院,大原030016

出　　处：《食品工业》2021年第3期120-124,共5页The Food Industry

基　　金：山西省重点研发计划(社会发展方面)项目(201903D321112)。

摘　　要：为优化超声提取美国黑莓花青素的工艺条件,在提取时间、提取温度、体积分数、液料比4个单因素试验基础上,按照Box-Behnken中心组合试验原理采用三因素三水平的响应面分析法,建立花青素提取工艺模型。结果表明,在乙醇体积分数88%、液料比23︰1 (mL/g)、提取温度37℃条件下,花青素含量最高,为2.61 mg/g。优化后的工艺有较高的准确性与实用性,可用于美国黑莓花青素提取。To optimize the condition for ultrasonic extraction of anthocyanin from American blackberry, the factors of extraction temperature and extraction time, ethanol content, liquid-solid ratio were optimized by single factor experiment. The design was made by the Box-behnken central combination test principle. The response surface method was used to investigate the following three factors three levels, to establish the extraction process model of anthocyanin. The results showed that under the condition of ethanol content 88%, liquid-solid ratio 23︰1(mL/g) and extraction temperature 37 ℃, the highest anthocyanin content of 2.61 mg/g was reached. The optimized process had high accuracy and practicability, which could be used to extract anthocyanin from American blackberry.

关 键 词：美国黑莓 花青素 响应面分析法 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]