Aptamer-quantum dots and teicoplanin-gold nanoparticles constructed FRET sensor for sensitive detection of Staphylococcus aureus  

作　　者：Xiaoqi Tao Ziyi Liao Yaqing Zhang Fei Fu Mengqi Hao Yang Song Erqun Song 

机构地区：[1]College of Pharmaceutical Sciences,Southwest University,Chongqing 400715,China [2]College of Food Science.Southwest University,Chongqing 400715,China

出　　处：《Chinese Chemical Letters》2021年第2期791-795,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.21974110,21575118,21976145 and 31672605);Natural Science Foundation Project of Chongqing (No.CSTC2019jcyj-msxmX0406)。

摘　　要：The detection of bacterial pathogen such as Staphylococcus aureus(S.aureus) is essential for the regulation of food hygiene and disease diagnosis.Herein,we developed a simple one-step fluorescence resonance energy transfer(FRET)-based sensor for specific and sensitive detection of S.aureus in food and serum samples,in which aptamer-modified quantum dots(aptamer-QDs) was employed as the energy donor and antibiotic of teicoplanin functionalized-gold nanoparticles(Teico-AuNPs) was chosen as the energy acceptor.Within 1 h,the FRET-based sensor showed a linear range of from 10 cfu/mL to 5 × 10^(8) cfu/mL,with the low limit of detection(LOD,2 cfu/mL) for S.aureus in buffer.When further applied to assay S.aureus in real samples,the FRET-based sensor showed good recoveries ranging from 84.5% to 110.0%,with relative standard derivations(RSDs) of 0.01%-0.44% and a LOD of 100 cfu/mL in milk,orange juice and human serum.

关 键 词：Fluorescence resonance energy transfer(FRET) Staphylococcus aureus APTAMER Quantum dot TEICOPLANIN Gold nanoparticles 

分 类 号：O657.3[理学—分析化学] TS207.4[理学—化学] R378.1[轻工技术与工程—食品科学]