2015-2019年北京市西城区人源性单核细胞增生李斯特菌的分子特征分析  

Molecular subtyping of Listeria monocytogenes isolated from patients in Xicheng district,Beijing,2015-2019

作　　者：霍哲[1] 张晓嫒[2] 徐俊[1] 曹玮[1] Huo Zhe;Zhang Xiaoai;Xu Jun;Cao Wei(Xicheng District Center of Disease Control and Prevention,Beijing 100120,China;Beijing Municipal Center for Disease Preventive Medical Research/Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning,Beijing 100013,China)

机构地区：[1]北京市西城区疾病预防控制中心,北京100120 [2]北京市疾病预防控制中心/北京市预防医学研究中心/食物中毒诊断溯源技术北京市重点实验室,北京100013

出　　处：《疾病监测》2021年第3期270-275,共6页Disease Surveillance

基　　金：北京市西城区优秀人才培养资助项目(No.20150051);首都卫生发展科研专项(首发No.2020-2-3012,No.2011-1013-02);北京市优秀人才培养资助青年骨干个人项目(No.2015000021469G186)。

摘　　要：目的研究北京市西城区人源性单核细胞增生李斯特菌(Lm)的血清学分型和分子特征。方法对2015—2019年北京市西城区40株Lm临床分离株进行多重PCR血清学分型、脉冲场凝胶电泳分型(PFGE)和多位点序列分型(MLST)。结果40例病例中,14例患者为妊娠感染相关病例,其中7例为新生儿感染,均治愈,无死亡病例。非妊娠感染相关病例中3例死亡。40株Lm共分为3种血清群,其中1/2a,3a为优势血清型。40株菌株分为26个型别,GX6A16.BJ0003型别为优势带型(9株)。GX6A16.BJ0003型别自2015—2019年连续5年均有检出,并存在2例死亡病例。GX6A16.BJ0014型别的2例病例均为妊娠感染相关病例。40株菌株被分为13个序列类型(STs),最常见的为ST8、ST87、ST5。结论2015—2019年北京市西城区人源性Lm以1/2a,3a为主要血清群,GX6A16.BJ0003型别和ST8为北京市西城区人源性Lm主要PFGE型别和MLST型别,存在同一克隆的菌株持续流行情况,应提高对高危型别的监测。Objective To study the serotype and molecular characteristics of Listeria monocytogenes isolated from patients in Xicheng district of Beijing from 2015 to 2019.Methods A total of 40 L.monocytogenes strains isolated from the patients from 2015 to 2019 were characterized by serotyping with multiplex PCR,pulsed field gel electrophoresis(PFGE)and multilocus sequence tying(MLST)analysis.Results In the 40 L.monocytogenes infection cases in this study,14 were pregnancy related infection cases.7 were neonatal infection cases,all were cured.In non-pregnancy related infection cases,3 were fetal.In the 40 L.monocytogenes isolates,3 serogroups were detected,in which 1/2 a,3 a was predominant.The 40 strains were divided into 26 different PFGE types,GX6 A16.BJ0003 was predominant(9 strains,22.5%),and GX6 A16.BJ0003 was detected in each year from 2015 to 2019,causing 2 deaths.The 2 cases associated with GX6 A16.BJ0014 were all pregnancy related infections.The 40 strains were divided into 13 ST types based on MLST typing,the most common ST was ST8,ST87 and ST5.Construction The predominant serogroup of L.monocytogenes isolated from patients in Xicheng district was 1/2 a,3 a from 2015 to 2019.GX6 A16.BJ0003 and ST8 were the predominant PFGE type and sequence type,respectively.There was persistence of spread of L.monocytogenes of same clone in Xicheng district.It is necessary to pay close attention the surveillance for high-risk pulse types.

关 键 词：单核细胞增生李斯特菌 血清型 脉冲场凝胶电泳 多位点序列分型 食源性致病菌 

分 类 号：R211[医药卫生—中医学] R446.5