喷他霉素的发酵培养基及工艺研究  

Fermentation Medium and Culture Process for the Production of Pentamycin

作　　者：谢秋萍 卫腾云 杨松柏[2] 吴远杰[2] 陈少欣[2] XIE Qiuping;WEI Tengyun;YANG Songbai;WU Yuanjie;CHEN Shaoxin(School of Chemistry and Chemical Engineering,Shanghai University of Engineering Science,Shanghai 201620;State Key Lab.of New Drug&Pharmaceutical Process,China state Institute of Pharmaceutical Industry,Shanghai Institute of Pharmaceutical Industry,Shanghai 201203)

机构地区：[1]上海工程技术大学化学化工学院,上海201620 [2]上海医药工业研究院,中国医药工业研究总院,创新药物与制药工艺国家重点实验室,上海201203

出　　处：《中国医药工业杂志》2021年第4期507-512,共6页Chinese Journal of Pharmaceuticals

摘　　要：本研究首先通过筛选获得了一株产喷他霉素的玫瑰藤黄链霉菌SIPI 2776-N1(产量为1.5 g/L),然后对其发酵的碳源和氮源进行筛选,并优化发酵培养基配方。结果表明,油脂对喷他霉素的合成有明显影响,棕榈油有利于产物的合成。摇瓶的发酵产量达到2.7 g/L。在5 L发酵罐中,通过调整培养基和采用分批补料的策略,使喷他霉素的产量达到5.8 g/L,为下一步工业化生产提供基础。In this study,a pentamycin-producing fungus,which was Streptomyce roseo luteus SIPI 2776-N1(the yield was 1.5 g/L),was obtained through screening.The carbon source and nitrogen source of its fermentation were screened,and the fermentation medium formula was optimized.The results showed that oil had a significant effect on the synthesis of pentamycin.Palm oil was conducive to the synthesis of products,and the fermentation yield of shake flasks reached 2.7 g/L.In the 5 L fermenter,through adjusting the medium and adopting the strategy of batch feeding,the output of pentamycin was reached to 5.8 g/L,which provided a basis for the next step of industrial production.

关 键 词：喷他霉素 玫瑰藤黄链霉菌 培养基优化 分批发酵 

分 类 号：TQ465.5[化学工程—制药化工] TQ927[轻工技术与工程—发酵工程]