敲低Aurora-A抑制HepG2人肝癌细胞增殖并促进其凋亡  

Knockdown of Aurora-A inhibits proliferation and promotes apoptosis of HepG2 hepatocellular carcinoma cells

作　　者：李忠贤 韩淑霞[2] 刘斌[3] 卜稳平 刘迪[4] 严慧[5] 刘清[4] LI Zhongxian;HAN Shuxia;LIU Bin;BU Wenping;LIU Di;YAN Hui;LIU Qing(Graduate School,Ningxia Medical University,General Hospital of Ningxia Medical University,Yinchuan 750004,China;Department of Gynecology,General Hospital of Ningxia Medical University,Yinchuan 750004,China;Comprehensive Training Center for Clinical Skills,General Hospital of Ningxia Medical University,Yinchuan 750004,China;Department of Hepatobiliary Surgery,General Hospital of Ningxia Medical University,Yinchuan 750004,China;Department of Oncology,General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学研究生学院,宁夏银川750004 [2]宁夏医科大学总医院妇科,宁夏银川750004 [3]宁夏医科大学总医院临床技能综合培训中心,宁夏银川750004 [4]宁夏医科大学总医院肝胆外科,宁夏银川750004 [5]宁夏医科大学总医院肿瘤内二科,宁夏银川750004

出　　处：《细胞与分子免疫学杂志》2021年第3期233-239,共7页Chinese Journal of Cellular and Molecular Immunology

基　　金：宁夏自然科学基金(NZ17141)。

摘　　要：目的探讨敲低Aurora-A对HepG2人肝癌细胞增殖和凋亡的影响。方法设计Aurora-A短发夹RNA(shRNA)片段,构建和包装Aurora-A shRNA慢病毒载体,转染HepG2细胞,实时定量PCR检测Aurora-A mRNA表达,Westernblot法检测Aurora-A蛋白及磷酸化水平,噻唑蓝(MTT)法检测细胞增殖,流式细胞术检测细胞凋亡。结果成功构建Aurora-A shRNA慢病毒载体,转染后的HepG2细胞Aurora-A蛋白磷酸化水平明显降低。敲低Aurora-A后,HepG2细胞增殖显著降低,细胞凋亡率显著增加。结论敲低Aurora-A的表达抑制HepG2细胞增殖并促进其凋亡。Objective To investigate the effects of knockdown of Aurora-A gene on the proliferation and apoptosis of HepG2 human hepatocellular carcinoma cells.Methods Aurora-A short hairpin RNA(Aurora-A shRNA)was designed and Aurora-A shRNA lentiviral vector was constructed and packed,and then transfected into HepG2 cells.Aurora-A mRNA expression was detected by real-time quantitative PCR.Aurora-A protein expression and phosphorylation level were detected by Western blotting.Cell proliferation was tested by MTT assay.Cell apoptosis was analyzed by flow cytometry.ResultsThe Aurora-A shRNA lentiviral vector was successfully constructed and Aurora-A protein phosphorylation level was significantly reduced in HepG2 cells transfected with the lentiviral vector.When Aurora-a was knocked down,the proliferation of HepG2 cells decreased and the apoptosis rate increased significantly.Conclusion Knockdown of Aurora-A can inhibit the proliferation and promote the apoptosis of HepG2 cells.

关 键 词：AURORA-A 细胞增殖 细胞凋亡 HEPG2人肝癌细胞 

分 类 号：Q279[生物学—细胞生物学] R734.2a[医药卫生—肿瘤]