盐酸-壳聚糖絮凝耦合处理回收鲢鱼糜漂洗液中的肌浆蛋白  

Hydrochloric acid-chitosan flocculation coupling treatment for recovery of sarcoplasmic protein from surimi rinsing solution of silver carp

作　　者：周文燕 黄琪琳[1] 翁武银 ZHOU Wenyan;HUANG Qilin;WENG Wuyin(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;College of Food and Biological Engineering,Jimei University,Xiamen 361013,China)

机构地区：[1]华中农业大学食品科学技术学院,武汉430070 [2]集美大学食品与生物工程学院,厦门361013

出　　处：《华中农业大学学报》2021年第3期264-270,共7页Journal of Huazhong Agricultural University

基　　金：现代农业产业技术体系专项(CARS-45-28)。

摘　　要：为提高鱼糜漂洗液中肌浆蛋白的利用率,以鲢鱼糜为研究对象,采用单一壳聚糖絮凝法和盐酸-壳聚糖絮凝耦合处理回收鲢鱼糜漂洗液中的肌浆蛋白,并通过单因素试验和Box-Bechnken中心组合设计对盐酸-壳聚糖絮凝耦合处理的絮凝条件进行优化。结果发现:盐酸处理(pH 3.0)可以显著提高壳聚糖作为单一絮凝剂时的肌浆蛋白回收率(从35.52%提高到81.54%),肌浆蛋白的最佳回收工艺确定为:pH 6.5,壳聚糖用量250 mg/L,温度30℃,时间90 min。在此条件下,可以获得85.23%的高蛋白质回收率以及73.32%的COD去除率。同时,此方法引入鱼糜漂洗液中的NaCl质量分数为0.006%,不会使后续废水处理成本增加,具有一定的可行性。The sarcoplasmic protein is a water-soluble protein,which accounts for 20%-40%of fish protein.It is lost with the rinsing liquid during the preparation of surimi,resulting in a waste of protein resources.In order to improve the utilization rate of sarcoplasmic protein in surimi rinsing solution,the single chitosan flocculation and hydrochloric acid-chitosan flocculation coupling treatment were used to recover the sarcoplasm protein from surimi rinsing solution of silver carp.The flocculation conditions of hydrochloric acid-chitosan flocculation coupling treatment were optimized through single factor experiments and Box-Bechnken central combination design.The results showed that the recovery rate of sarcoplasmic protein was significantly increased from 35.52%to 81.54%by hydrochloric acid treatment(pH 3.0)when chitosan was used as a single flocculant.The recovery process of sarcoplasmic protein was optimized as follows:pH 6.5,250 mg/L chitosan,30℃,90 min.Under these conditions,a high protein recovery rate of 85.23%and a COD removal rate of 73.32%was obtained.The NaCl of 0.006%introduced into the surimi rinsing liquid in this method does not increase the cost of subsequent wastewater treatment,and has a certain feasibility.It will provide a theoretical basis for recovering and utilizing sarcoplasmic protein in surimi rinsing water,and a technical support and reference for purifying rinsing wastewater during the process of surimi.

关 键 词：鲢 盐酸处理 肌浆蛋白 壳聚糖 絮凝 鱼糜漂洗液 废水处理 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]