顶空-气相质谱联用技术分析凉粉草挥发性成分  

Analysis of Volatile Constituents from Mesona chinensis Benth. by HS-GC-MS

作　　者：卢晓莹 林翠清 赖志明 江晓鑫 严萍[1] 詹若挺[1] LU Xiao-Ying;LIN Cui-Qing;LAI Zhi-Ming;JIANG Xiao-Xin;YAN Ping;ZHAN Ruo-Ting(Research Center of Chinese Herbal Resource Science and Engineering of Guangzhou University of Chinese Medicine,Key Laboratory of Chinese Medicinal Resource from Lingnan Guangzhou University of Chinese Medicine,Ministry of Education,Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines,Guangzhou 510006 Guangdong,China;Guangdong Nanling Pharmaceutical Co.Ltd,Pingyuan 514600 Guangdong,China)

机构地区：[1]广州中医药大学中药资源科学与工程研究中心、岭南中药资源教育部重点实验室(广州中医药大学),国家中成药工程技术研究中心南药研发实验室,广东广州510006 [2]广东南领药业有限公司,广东平远514600

出　　处：《广州中医药大学学报》2021年第5期1025-1031,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广州中医药大学2017年高水平大学建设面上项目(广中医研[2017]10号);广东省现代农业产业技术体系创新团队建设项目——南药产业(编号:2019KJ148);广东省乡村振兴战略专项“发展南药一村一品、一镇一业产业支撑”项目(粤农农计[2019]5号);校级大学生创新创业训练计划项目(编号:201910572266)。

摘　　要：【目的】分析不同加工方式和不同栽培品种凉粉草挥发性成分差异。【方法】采用顶空-气相色谱质谱联用(HS-GC-MS)技术测定分析不同凉粉草样品挥发性成分,分析结果通过质谱标准库检索,并采用面积归一化法计算相对质量分数,比较不同样品挥发性成分差异。【结果】从台湾凉粉草和本地凉粉草中共鉴别出24种挥发性成分,主要为烯类成分。2种凉粉草中均含有较大比例的石竹烯,台湾凉粉草中含12.9%,本地凉粉草中含34.2%。台湾凉粉草中特有成分α-芹子烯、β-芹子烯和α-红没药醇等共占25.5%。微发汗处理对凉粉草中石竹烯类成分的影响较大。【结论】建立的HS-GC-MS联用技术可对凉粉草的挥发性成分进行快速、有效的定性分析,且本地凉粉草与台湾引种的凉粉草挥发油特征成分差异较大,可为其鉴别提供参考。Objective To analyze the differences in volatile constituents from cultivated varieties of Mesona chinensis Benth. by different process methods. Methods Headspace gas-chromatography-mass spectrum(HS-GCMS)was used to analyze the volatile components from different samples of Mesona chinensis Benth.. The achieved analysis results were confirmed by mass spectral library searching,and the relative mass fraction was calculated by area normalization method, so as compare the differences in the volatile constituents from varieties of Mesona chinensis Benth.. Results A total of 24 kinds of volatile constituents,mainly alkenes,were identified from Taiwan Mesona chinensis Benth. and local Mesona chinensis Benth.. Both kinds of Mesona chinensis Benth. contained a large proportion of caryophyllenes,which were 12.9% and 34.2% in Taiwan plant and local plant,respectively. The specific constituents such as α-Selinene,β-Selinene,and α-Bisabolol from Taiwan Mesona chinensis Benth.accounted for 25.5%. The micro-sweat treatment had a greater impact on caryophyllene constituents in Mesona chinensis Benth.. Conclusion HS-GC-MS established is quickly effective for qualitative analysis of the volatile constituents of Mesona chinensis Benth.. There are greater differences in characterstic volatile constituents from locol Mesona chinensis Benth. and Taiwan Mesona chinensis Benth., which can provide a reference for their identification.

关 键 词：凉粉草 台湾引种 顶空-气相质谱联用 挥发性成分 石竹烯 

分 类 号：R282.2[医药卫生—中药学]