Improved osteointegration and angiogenesis of strontium-incorporated 3D-printed tantalum scaffold via bioinspired polydopamine coating  

作　　者：Shi Cheng Jin Ke Mengyu Yao Hongwei Shao Jielong Zhou Ming Wang Xiongfa Ji Guoqing Zhong Feng Peng Limin Ma Yu Zhang 

机构地区：[1]Department of Orthopedics,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510080,China [2]The Second School of Clinical Medicine,Southern Medical University,Guangzhou 510515,China

出　　处：《Journal of Materials Science & Technology》2021年第10期106-118,共13页材料科学技术（英文版）

基　　金：the National Natural Science Foundation of China(Nos.31700880 and 81972126);the China Postdoctoral Science Foundation(No.2019M662830);the Natural Science Foundation of Guangdong Province,China(No.2020A1515010827 and 2018A030313709);the Science and Technology Planning Project of Guangzhou City(No.201803010106);the High-level Hospital Construction Project(No.KJ012019100)。

摘　　要：Tantalum(Ta)is used in orthopedic implants because it has excellent biocompatibility.However,high elastic modulus,bio-inertness,and unsatisfactory osteointegration limits its wider use in clinical applications.Herein,a 3 D porous Ta scaffold with low elastic modulus was fabricated using selective laser melting(SLM).Strontium(Sr)was incorporated on the surface of the scaffold with the aid of polydopamine(PDA)to further improve its osteointegration ability.The prepared scaffolds exhibited a stable Sr ion release in 14 d.Rat bone marrow stem cells(BMSCs)showed improved early adhesion and spreading after Sr was incorporated on the porous Ta surface.The osteogenic behavior,including extracellular matrix mineralization(ECM),alkaline phosphatase activity(ALP),and expression of bone-related RNA,were all enhanced.Furthermore,the Sr-incorporated porous Ta scaffolds exhibited better angiogenic behavior,such as promoting migration,tube formation,and angiogenesis-related RNA expression abilities of human vascular endothelial cells(HUVECs).Additionally,histological images(H&E,Masson and CD31 immunofluorescent staining)suggested that Sr-incorporated porous Ta scaffolds displayed enhanced osteointegration and angiogenesis after implantation in rat femur for 12 weeks.These findings prove that the PDA-based Sr-incorporated porous Ta scaffolds show promising use in orthopedic implants.

关 键 词：3D Ta scaffold Strontium OSTEOGENESIS ANGIOGENESIS Orthopedic implant 

分 类 号：R318.08[医药卫生—生物医学工程] TG146.416[医药卫生—基础医学] TP391.73[一般工业技术—材料科学与工程]