富硒黑木耳多糖的理化性质及抗氧化活性研究  

Research on Physicochemical Properties and Antioxidant Activity of Selenium-enriched Auricularia auricula Polysaccharides

作　　者：吕明帅 赵博[1] 孙文玉 万水晶 刘春延[1] 张国财[1] LV Ming-shuai;ZHAO Bo;SUN Wen-yu;WAN Shui-jing;LIU Chun-yan;ZHANG Guo-cai(College of Forestry,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《中国调味品》2021年第6期54-59,共6页China Condiment

基　　金：中央财政林业科技推广示范资金项目([2019]HZT02);哈尔滨市应用技术研究与开发项目(2016RAXXJ035)。

摘　　要：采用超声辅助法对富硒黑木耳多糖和普通黑木耳多糖进行提取和分离纯化,得到富硒黑木耳多糖(Se-AAP-2)和普通黑木耳多糖(AAP-2),比较分析Se-AAP-2和AAP-2的分子量、单糖组成、纯度、糖醛酸含量、体外及体内抗氧化活性。结果表明,Se-AAP-2和AAP-2均由甘露糖和葡萄糖组成,分子摩尔比分别为1∶1.31和1∶1.28;相比AAP-2,Se-AAP-2的平均分子量降低了23.5%(P<0.01),糖醛酸含量提高了33.7%(P<0.01),说明富硒能够显著降低黑木耳多糖的分子量,提升其糖醛酸含量。此外,Se-AAP-2拥有更高的体外抗氧化活性,相比AAP-2,其总还原力提高了11.4%,羟基自由基和ABTS自由基清除能力IC 50亦分别降低了42.2%和26.4%。体内抗氧化活性评价结果表明,Se-AAP-2相较于AAP-2具有更强的体内抗氧化活性,能够显著提高小鼠肝、肾、心、血清中T-SOD、GSH-Px的活性,降低其MDA含量。The polysaccharides of Se-AAP-2 and AAP-2 are extracted and purified by ultrasonic-assisted method.The molecular weight,monosaccharide composition,purity,uronic acid content and antioxidant activity in vitro and in vivo of Se-AAP-2 and AAP-2 are compared and analyzed.The results show that Se-AAP-2 and AAP-2 are both composed of mannose and glucose,and the molecular molar ratio is 1∶1.31 and 1∶1.28 respectively;compared with AAP-2,the average molecular weight of Se-AAP-2 is reduced by 23.5%(P<0.01),and the uronic acid content is increased by 33.7%(P<0.01),indicating that selenium enrichment could significantly reduce the molecular weight of Auricularia auricula polysaccharides and increase the uronic acid content.In addition,Se-AAP-2 has higher antioxidant activity in vitro.Compared with AAP-2,its total reducing power is increased by 11.4%,and the IC 50 of hydroxyl radical and ABTS radical scavenging ability is also decreased by 42.2%and 26.4%respectively.The evaluation results of antioxidant activity in vivo show that Se-AAP-2 has stronger antioxidant activity in vivo than AAP-2,it could significantly increase the activity of T-SOD and GSH-Px in the liver,kidney,heart and serum of mice and reduce its MDA content.

关 键 词：黑木耳 硒 多糖 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]