依普黄酮对骨质疏松大鼠颌骨成骨细胞的线粒体相关因子的影响  

Effects of ipriflavone on the mitochondrial related factors of osteoblasts from the jaws of osteoporotic rats

作　　者：于书娟 刘洪臣[2] YU Shu-juan;LIU Hong-chen(Stomatology Department,960th Hospital of PLA,Shandong 250031,China;Institute of Stomatology&Oral Maxilla Facial Key Laboratory,Chinese PLA General Hospital,Beijing 100853,China)

机构地区：[1]中国人民解放军第九六〇医院口腔科,山东250031 [2]解放军总医院口腔医学研究所,口腔颌面战创伤军队重点实验室,口腔颌面修复军队医学重点实验室,北京100853

出　　处：《口腔颌面修复学杂志》2021年第3期161-165,240,共6页Chinese Journal of Prosthodontics

摘　　要：目的:探讨依普黄酮对骨质疏松大鼠颌骨成骨细胞线粒体相关因子ATP、ROS和UCP2的影响。方法:大鼠通过去势法建立骨质疏松的动物模型。培养颌骨成骨细胞,分为两组(依普黄酮组和对照组),采用含10-8M依普黄酮的培养基和不含依普黄酮的培养基培养成骨细胞72h。分别进行ATP含量检测、ROS含量检测、UCP2的基因检测和UCP2的蛋白检测。结果:与对照组相比,在依普黄酮组,骨质疏松大鼠颌骨成骨细胞的ATP含量增加,ROS含量减少,UCP2的mRNA水平和蛋白水平均出现了增加。结论:在骨质疏松大鼠颌骨成骨细胞的ATP、ROS和UCP2的表达调控上,依普黄酮可能发挥着重要的作用。Objective:To discuss the influence of iprflavone on mitochondrial related factors:uncoupling protein 2(UCP2),adenosine triphosphate(ATP)and reactive oxygen species(ROS)of the osteoblasts derived from the jaws of osteoporotic rats.Methods:An animal model of osteoporosis was established by ovariectomy in rats.Osteoblasts from the jaws were cultured and divided into two groups(the ipriflavone group and the control group).Then the cells were cultured in the medium containing 10-8 M ipriflavone and the medium without ipriflavone for 72 h.ATP content,ROS content,the expression of UCP2 gene and protein were detected respectively.Results:Compared with the control group,in the ipriflavone group,ATP content and the expression level of UCP2 mRNA and protein were increased,ROS content was decreased.Conclusion:ipriflavone may play an important role in the regulation of UCP2,ROS and ATP.

关 键 词：成骨细胞 依普黄酮 UCP2 ROS ATP 

分 类 号：R780.2[医药卫生—口腔医学]