外周血干细胞样记忆性T细胞在肾癌患者中的表达水平及临床意义  

Expression and Clinical Significance of Peripheral Blood Stem-like Memory T Cells in Patients with Renal Cell Carcinoma

作　　者：陈婷 黄小雨 杨栋梁 孙方浒 蒋晓东 Chen Ting;Huan Xiaoyu;Yang Dongliang(Graduate School of Xuzhou Medical University,Jiangsu 221004,China)

机构地区：[1]徐州医科大学,221004 [2]徐州医科大学附属连云港医院,222000

出　　处：《医学研究杂志》2021年第5期76-79,137,共5页Journal of Medical Research

基　　金：江苏省研究生实践创新计划项目(SJCX190943)。

摘　　要：目的研究肾癌患者外周血干细胞样记忆性T细胞(stem-like memory T cells,TSCM)的分布特征,探讨TSCM与临床病理特征的关系。方法选取2019年1月~2020年3月连云港第一人民医院收治的65例肾癌患者为肾癌组,同期30例健康志愿者为健康组。运用流式细胞术检测两组患者外周血中初始T细胞(naive T cells,TN)和TSCM比例,比较肾癌组与健康组的差异,分析TSCM、TN和TSCM/TN值与临床病理特征的关系。结果与健康组比较,肾癌组患者外周血TSCM比例(0.15%±0.06%vs 0.20%±0.11%,t=2.524,P<0.05)和TN比例(2.66%±1.18%vs 6.29%±1.58%,t=12.499,P<0.01)均明显降低,TSCM/TN值(6.48%±3.70%vs 3.31%±1.82%,t=5.600,P<0.01)明显升高。肾癌患者中,TSCM比例与肿瘤TNM分期(r=-0.258,P<0.05)和Fuhrman核分级(r=-0.271,P<0.05)呈负相关;TN比例与肿瘤TNM分期(r=-0.447,P<0.01)和Fuhrman核分级(r=-0.263,P<0.05)呈负相关;TSCM/TN值与肿瘤TNM分期(r=0.433,P<0.01)呈正相关。结论肾癌患者外周血TSCM、TN及TSCM/TN值与肿瘤TNM分期和Fuhrman核分级密切相关,可用于评估肿瘤发生、发展情况,TSCM在肾癌的过继性免疫治疗中具有广阔的应用前景。Objective To study the expression of peripheral blood stem-like memory T cells(TSCM)in patients with renal cell carcinoma and to analyze their relationship to clinicopathological characteristics.Methods From January 2019 to March 2020,there were 65 patients with renal cell carcinoma in the First People′s Hospital of Lianyungang served as renal cell carcinoma group and 30 healthy volunteers as healthy group.The percentages of naive T cells(TN)and TSCM in the peripheral blood were detected by flow cytometry and the differences between the two groups were compared.The relationship of clinicopathological features to TSCM,TN and TSCM/TN ratio was analyzed.Results Compared with healthy group,the percentages of TSCM(0.15%±0.06%vs 0.20%±0.11%,t=2.524,P<0.05)and TN(2.66%±1.18%vs 6.29%±1.58%,t=12.499,P<0.01)in peripheral blood of the renal cell carcinoma patients decreased significantly,while the ratio of TSCM to TN(6.48%±3.70%vs 3.31%±1.82%,t=5.600,P<0.01)increased significantly.The proportion of TSCM was negatively correlated with tumor TNM stage(r=-0.258,P<0.05)and Fuhrman grade(r=-0.271,P<0.05).The proportion of TN was negatively correlated with tumor TNM stage(r=-0.447,P<0.01)and Fuhrman grade(r=-0.263,P<0.05).The ratio of TSCM to TN was positively correlated with tumor TNM stage(r=0.433,P<0.01).Conclusion TSCM,TN and the ratio of TSCM to TN in peripheral blood of patients with renal cell carcinoma are closely related to tumor TNM stage and Fuhrman grade,which could be used to evaluate the occurrence and development of tumor.TSCM might have important clinical application value in adaptive or engineered cell immunotherapy of renal cell carcinoma.

关 键 词：肾癌 干细胞样T细胞 初始T细胞 T淋巴细胞亚群 细胞免疫 

分 类 号：R73[医药卫生—肿瘤]