采用DNA条形码技术鉴别面膜粉中常用的药妆同源植物源性成分  

Identification of the common cosmeceutical homologous plants in mask powder products based on DNA barcoding

作　　者：王洪越 王迎春 江丽[1] 杨艳歌[1] 刘鸣畅[1] 吴亚君[1] WANG Hong-yue;WANG Ying-chun;JIANG Li;YANG Yan-ge;LIU Ming-chang;WU Ya-jun(Chinese Academy of Inspection and Quarantine,Beijing 100176,China)

机构地区：[1]中国检验检疫科学研究院,北京100176

出　　处：《日用化学工业》2021年第6期528-534,共7页China Surfactant Detergent & Cosmetics

基　　金：中国检验检疫科学研究院基本科研业务费资助项目(2020JK046,2018JK030)。

摘　　要：以市面上常用于面膜粉的22种药妆同源植物原料为研究对象,比较了常用的5组基因的物种鉴别效果,筛选出适用于面膜粉中药妆同源植物源性成分鉴别的DNA条形码。结果表明:ITS 2/3和mat K基因联合能够准确的鉴定药妆同源植物源性成分。同时采用该方法,结合本实验室建立的混合样品克隆测序流程,对14份市售样品(包括11份单一样品,3份混合产品)进行了适用性分析。实验结果显示面膜粉中的药妆同源植物成分存在35.71%的掺假率。本研究建立的基于ITS 2/3和mat K基因的DNA条形码技术能够准确的鉴定面膜粉中的药妆同源植物源性成分,为该类产品的质量和安全提供技术支撑。Took 22 common cosmeceutical plant ingredients in mask powder products as the research object,and screened out the most suitable genes for species identification from five genes.The results show that combined ITS 2/3 and matK can accurately identify cosmeceutical homologous plants.Furthermore,combined with the clone sequencing process for mixture established by our laboratory,14 commercial mask powder products(including 11 single samples and 3 mixed products)are used to analyze the applicability.The results show that the adulteration rate is 35.71%in the cosmetics and edible homologous plant in mask powder products.The establishment of DNA barcoding based on ITS 2/3 and matK genes can accurately and reliably identify cosmeceutical homologous plants,which provides technical support for the quality and safety control of mask powder products.

关 键 词：面膜粉 药妆同源植物 DNA条形码 质量 安全 

分 类 号：TQ658[化学工程—精细化工]