气相色谱法同时快速测定大豆油脱臭馏出物中的生育酚、植物甾醇和角鲨烯含量  

Simultaneous and rapid determination of tocopherol,phytosterol and squalene in soybean oil deodorized distillate by gas chromatography

作　　者：甘欢华 张斌 刘钟栋[1] 李华[1] 魏国华 GAN Huanhua;ZHANG Bin;LIU Zhongdong;LI Hua;WEI Guohua(School of Food Science and Technology,Henan University of Technology,Zhengzhou 450000,China;Yichun Big Turtle Life Science Co.,Ltd.,Yichun 336000,Jiangxi,China)

机构地区：[1]河南工业大学粮油食品学院,郑州450000 [2]宜春大海龟生命科学有限公司,江西宜春336000

出　　处：《中国油脂》2021年第5期39-42,70,共5页China Oils and Fats

摘　　要：建立了可以同时定量分析大豆油脱臭馏出物中生育酚(α-生育酚、(β+γ)-生育酚、δ-生育酚)、植物甾醇(菜籽甾醇、菜油甾醇、豆甾醇、β-谷甾醇)和角鲨烯含量的气相色谱检测方法。以十六酸十六醇酯为内标物质,样品直接溶解在内标溶液中,采用HP-1色谱柱分离,以内标法定量。结果表明:各组分之间分离度良好,生育酚、植物甾醇和角鲨烯分别在0.4~6.4 mg/mL、0.5~8.0 mg/mL、0.4~4.0 mg/mL范围内呈现良好的线性关系,R^(2)均大于等于0.999,平均加标回收率分别为95.38%~102.78%、96.40%~101.24%、96.14%~104.43%,相对标准偏差均小于10%,检出限分别为0.08、0.15、0.05 mg/L。该方法前处理简单、重复性好、灵敏度高、结果准确可靠,可应用于大豆油脱臭馏出物中生育酚、植物甾醇、角鲨烯含量的同时测定。A gas chromatography detection method was established,which could simultaneously quantitatively analyze the tocopherols(α-tocopherol,(β+γ)-tocopherol,δ-tocopherol),phytosterols(rapeseed sterol,campesterol,stigmasterol,β-sitosterol)and squalene in soybean oil deodorized distillate.With cetyl palmitate as the internal standard substance,the sample was directly dissolved in the internal standard solution,and the HP-1 column was used to separate various components.The results showed that the separation of various components was good.The tocopherol,phytosterol and squalene showed good linear relationships in the ranges of 0.4-6.4 mg/mL,0.5-8.0 mg/mL and 0.4-4.0 mg/mL respectively,the relative coefficients were all greater than or equal to 0.999,and their average recovery rates were 95.38%-102.78%,96.40%-101.24%,96.14%-104.43%respectively.The relative standard deviations were all lower than 10%,and their detection limits were 0.08,0.15 mg/L and 0.05 mg/L,respectively.The method had the items of simple pretreatment,good reproducibility,high sensitivity,and accurate and reliable results,which could be applied in the simultaneous determination of tocopherol,phytosterol and squalene in soybean oil deodorized distillate.

关 键 词：大豆油脱臭馏出物 气相色谱法 同时测定 生育酚 植物甾醇 角鲨烯 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ646[轻工技术与工程—食品科学与工程]