5类食品中叶酸的检测及稀释倍数估算  

Detection of folic acid and estimation of dilution multiple in 5 kinds of food

作　　者：任新军 肖萌 王颖 辛燕斌 米容融 REN Xin-Jun;XIAO Meng;WANG Ying;XIN Yan-Bin;MI Rong-Rong(Yunnan Institute of Product Quality Supervision and Inspection,Kunming 650223,China;School of Ethnic Medicine,Yunnan Minzu University,Kunming 650504,China)

机构地区：[1]云南省产品质量监督检验研究院,昆明650223 [2]云南民族大学民族医药学院,昆明650504

出　　处：《食品安全质量检测学报》2021年第8期3335-3340,共6页Journal of Food Safety and Quality

基　　金：云南省科技创新平台建设计划项目(2015DH022)。

摘　　要：目的解决GB5009.211—2014《食品安全国家标准食品中叶酸的测定》检测食品中叶酸时,试样稀释液浓度偏离的问题。方法实测5类常见食品的叶酸含量,归纳试样稀释方法,推导稀释倍数估算公式,验证理论稀释倍数f在实测中的应用。结果测得植物产品中叶酸的质量分数在5.03~287.3μg/100 g之间,含量差异较大,试样稀释5~50倍;肉类食品中叶酸在11.1~65.4μg/100g之间,稀释5~20倍;婴幼儿辅食营养包中叶酸在653.2~1024.4μg/100g之间,可以按100倍稀释;婴幼儿配方乳粉中叶酸为91.8~143.5μg/100g,稀释10~25倍;维生素片中叶酸含量很高,定容后需要稀释8000~20000倍。结论试样的稀释可以通过稀释倍数估算公式进行估算,结合稀释梯度,可大幅提高有效检测的概率,其中植物产品需要设置0.5f、1f、2f三个稀释梯度。稀释倍数估算和稀释梯度设置可以减少反复实验,提高检测效率。Objective To solve the problem of concentration deviation of sample dilution for detection of folic acid in food by GB 5009.211—2014 National food safety standard-Determination of folic acid in food.Methods The content of folic acid in 5 kinds of common foods was detected and the sample dilution method was summarized.Estimation formula of dilution multiple was deduced and the application of theoretical dilution multiple f in actual measurement was verified.Results The folic acid in vegetable food was detected as 5.03-287.3μg/100 g with a large difference and the dilution of the samples was 5-50 times.Folic acid in meat was 11.1-65.4μg/100 g with a dilution of 5-20 times.The content in infant supplementary nutrition package was 653.2-1024.4μg/100 g which could be diluted by 100 times.Folic acid in infant formula milk powder was between 91.8 and 143.5μg/100 g with 10-25 times dilution.The content of folic acid in vitamin tablets was very high so that it needed a dilution of 8000-20000 times.Conclusion Dilution of samples can be estimated by estimation formula of dilution multiple.Combined with the dilution gradient,the probability of effective detection can be greatly improved.Among them,vegetable food needs to set 3 kinds of dilution gradients of 0.5 f,1 f,and 2 f.Dilution multiple estimation and dilution gradient setting can reduce repeated experiments and improve detection efficiency.

关 键 词：叶酸 微生物法 稀释 

分 类 号：TS207.3[轻工技术与工程—食品科学]