Advances in aptamer-based nanomaterials for separation and analysis of non-genetic biomarkers in biofluids  

作　　者：Zidan Wang Chuwen Huang Nianrong Sun Chunhui Deng 

机构地区：[1]Department of Gastroenterology,Zhongshan Hospital of Fudan University,Shanghai 200032,China [2]Department of Chemistry,Fudan University,Shanghai 200032,China

出　　处：《Science China Chemistry》2021年第6期932-947,共16页中国科学（化学英文版）

基　　金：supported by the National Key R&D Program of China(2018YFA0507501);the National Natural Science Foundation of China(22074019,22004017);Shanghai Sailing Program(20YF1405300)。

摘　　要：Body fluids contain a large number of disease markers including proteins,exosomes and cells,the concentration of which indicates the health status of a body system and can be hence applied to predict the degree of seriousness of a disease.However,the content of disease markers is relatively low.For solving this problem,a variety of pretreatment methods have been developed,among which aptamer-based nanomaterials hold great potential for biomarker capture because of various natural properties of nano-substrates such as high specific surface area,as well as the high affinity of aptamer towards target and the higher stability than antibody.In this review,we summarized the recent aptamer-based nanomaterials for the separation and analysis of different biomarkers including proteins,exosomes and cells.More specifically,the standard of classification description was based on the applied substrates including magnetic substrates and non-magnetic substrates.Moreover,the perspective of aptamer-based nanomaterials was discussed.

关 键 词：aptamers NANOMATERIALS PROTEINS cells EXOSOMES biomarkers 

分 类 号：R318.08[医药卫生—生物医学工程] TB383.1[医药卫生—基础医学]