微生物非核糖体多肽的合成基因簇挖掘及其合成研究进展  

Advances in biosynthetic gene cluster mining and biosynthesis of microbial non-ribosomal peptides

作　　者：魏小雅 刘欣 于宏伟[1] 郭润芳[1] Wei Xiao-ya;Liu Xin;Yu Hong-wei;Guo Run-fang(College of Food Science and Technology,Hebei Agricultural University,Baoding 071000)

机构地区：[1]河北农业大学食品科技学院,保定071000

出　　处：《中国抗生素杂志》2021年第5期353-361,共9页Chinese Journal of Antibiotics

基　　金：河北省自然基金项目(No.C2018043);河北农业大学大学生创新创业训练计划资助项目(No.201910086015)。

摘　　要：非核糖体多肽因其独特的生物活性受到关注,它结构复杂、种类繁多。许多微生物能利用非核糖体肽合成酶(nonribosomal peptide synthase,NRPSs)合成这些非核糖体多肽。NRPSs是一类分子巨大的蛋白复合物,由一系列催化结构域组成,能识别、激活、转运氨基酸底物并按特定顺序合成非核糖体肽(non-ribosomal peptide,NRPs)。其模块化结构涉及的基因在基因组上成簇排列。基于基因组挖掘技术可以预测基因组上的次级代谢产物合成基因簇,并发现新的次级代谢产物。针对非核糖体多肽的生物合成基因簇的分布、挖掘、模块化结构以及合成等方面的研究进行分析,有望为理解非核糖体肽合成酶系统合成生物活性物质的机制和挖掘新型生物活性物质提供依据。Non-ribosomal peptides have attracted much attention because of their unique biological activities.They have complex structures and a wide variety of peptides.Many microorganisms can synthesize these non-ribosomal peptides using non-ribosomal peptide synthetase(NRPSs).NRPSs is a large class of protein complexes,which is composed of a series of catalytic domains.It can recognize,activate,transport amino acid substrates and synthesize non-ribosomal peptides(NRPs)in a specific sequence.The genes involved in its modular structure are arranged in clusters on the genome.Based on the technology of genome mining,the synthetic gene clusters of secondary metabolites in the genome can be predicted and new secondary metabolites can be found.The distribution,mining,modular structure and synthesis of non-ribosomal peptide biosynthesis gene clusters are analyzed,which is expected to provide a basis for understanding the mechanism of biosynthesis of bioactive substances and mining new bioactive substances in non-ribosomal peptide synthase system.

关 键 词：非核糖体多肽 非核糖体肽合成酶 基因簇 生物合成 

分 类 号：Q936[生物学—微生物学]