新疆薰衣草精油抗氧化活性成分的组效关系研究  被引量:13

Study on the Composition-activity Relationship of the Antioxidant Active Component in Essential Oil of Lavandula angustifolia from Xinjiang

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作  者:陈萍 刘兵 符继红[1] CHEN Ping;LIU Bing;FU Jihong(School of Chemical Engineering and Technology,Xinjiang University,Urumqi 830046,China)

机构地区:[1]新疆大学化工学院,乌鲁木齐830046

出  处:《中国药房》2021年第12期1460-1465,共6页China Pharmacy

基  金:新疆维吾尔自治区自然科学基金资助项目(No.2020D01C032);国家自然科学基金资助项目(No.21565024)。

摘  要:目的:探讨薰衣草精油抗氧化活性成分的组效关系。方法:采用1,1-二苯基-2-三硝基苯(DPPH)自由基清除法评价薰衣草精油的抗氧化活性;采用气质联用技术(GC-MS)结合保留指数对薰衣草精油成分进行定性分析;采用主成分分析、偏最小二乘法分析薰衣草精油化学成分与抗氧化活性的关系,以变量投影重要性(VIP)>1筛选对DPPH自由基消除有较大贡献的成分。结果:3个不同品种共9批薰衣草精油的平均半抑制浓度(IC50)为4.82~9.88 mg/mL,法国蓝、H-701、Xinxun-4精油样品的平均IC50分别为6.66~8.58、4.82~7.73、9.55~9.88 mg/mL;从中共鉴定出40个化学成分。PCA分析结果显示,前二个主成分的累积方差贡献率为81.8%;9批样品被分为3个区域,不同品种各归为一类;法国蓝薰衣草精油的特征变量成分为峰32对应的乙酸薰衣草酯、峰9对应的反式-β-罗勒烯;H-701蓝薰衣草精油的特征变量成分为峰16对应的芳樟醇、峰30对应的乙酸芳樟酯和峰21对应的萜品烯-4-醇;Xinxun-4蓝薰衣草精油的特征变量成分为峰8对应的桉树脑、峰18对应的樟脑和峰20对应的2-茨醇。薰衣草精油中对抗氧化活性贡献较大的成分为峰16对应的芳樟醇(VIP值为2.940 0)、峰21对应的萜品烯-4-醇(VIP值为2.863 1)、峰35对应的石竹烯(VIP值为2.570 8)、峰8对应的桉树脑(VIP值为2.115 8)。结论:薰衣草精油具有一定的抗氧化活性,H-701精油样品的抗氧化活性较强;峰16对应的芳樟醇可能是薰衣草精油消除DPPH自由基贡献最大的成分。OBJECTIVE:To investigate the composition-activity relationship of the antioxidant active component in essential oil of Lavandula angustifolia from Xinjiang.METHODS:Antioxidant activity of essential oil of L.angustifolia was evaluated with1,1-diphenyl-2-picrylhydrazyl(DPPH)free radicals scavenging method.GC-MS combined with retention index were adopted to qualitatively analyze the compounds.The relationship of chemical components with antioxidant activity of essential oil of L.angustifolia was analyzed by principal component analysis(PCA)and partial least squares analyses(PLS).The variable importance in projection(VIP>1)was used to screen the components which had great contribution to the elimination of DPPH free radical.RESULTS:The IC50 values of essential oil in 9 batches of L.angustifolia from 3 different varieties was 4.82-9.88 mg/m L,IC50 of France blue was 6.66-8.58 mg/mL,IC50 of H-701 and Xinxun-4 were 4.82-7.73 mg/mL and 9.55-9.88 mg/mL,respectively.A total of 40 chemical components were identified of essentil oil in 9 batches of L.angustifolia.Results of PCA analysis showed that accumulative variance contribution rate of former 2 main components was 81.8%;9 batches of samples were divided into 3 regions,and different varieties were classified into one category;the characteristic variable components of the essential oil of French blue samples were lavandulyl acetate corresponding to peak 32 and trans-β-ocimene corresponding to peak 9;the characteristic variable components of the essential oil of H-701 samples were linalool corresponding to peak 16,linalyl acetate corresponding to peak 30 and terpinen-4-ol corresponding to peak 21;the characteristic variable components of the essential oil of Xinxun-4 samples were eucalyptol corresponding to peak 8,camphor corresponding to peak 18 and 2-borneol corresponding to peak 20.The compound with the greatest antioxidant activity was linalool corresponding to peak 16(VIP=2.9400),followed by terpenein-4-ol corresponding to peak 21(VIP=2.8631),caryophyllene correspond

关 键 词:薰衣草精油 抗氧化活性 气质联用技术 组效关系 

分 类 号:R284.1[医药卫生—中药学] R285[医药卫生—中医学]

 

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