富硒长双歧杆菌DD98对X射线所致放射性肝损伤小鼠的保护作用  

Protective Effects of Selenium-enriched Bifidobacterium longum DD98 on X-Ray Radiation-induced Hepatic Tissueinjury in Mice

作　　者：陆春怡 高斐 朱慧 钱志祥 杨萍 李振 毛文伟[1] 殷瑜[1] 陈代杰[1] LU Chun-yi;GAO Fei;ZHU Hui;QIAN Zhi-xiang;YANG Ping;LI Zhen;MAO Wen-wei;YIN Yu;CHEN Dai-jie(School of Pharmacy,Shanghai Jiaotong University,Shanghai 201100,China)

机构地区：[1]上海交通大学药学院,上海201100

出　　处：《现代食品科技》2021年第6期10-19,共10页Modern Food Science and Technology

基　　金：国家自然科学基金项目(81872775);上海市科技人才计划项目(17XD1423200)。

摘　　要：本研究旨在讨论富硒长双歧杆菌DD98(Selenium-enriched Bifidobacterium longum DD98,Se-DD98)对X射线所致发射性肝损伤小鼠的保护作用。雄性SPF级balb/c小鼠随机分为5组:正常对照组、辐射对照组、Se-DD98高剂量组、Se-DD98低剂量、DD98对照组,连续给药28 d并监测体重。第29 d采用X射线全身照射,实验结束后进行指标检测。与辐射对照组的体重(24.44 g)和肝脏指数(4.49%)比较,Se-DD98高剂量组体重(26.96 g)及肝脏指数(5.10%)显著改善、血清肝功能指标(ALT、AST)和体内氧化压力水平(MDA、LDH)显著降低,抗氧化酶活力(CAT、SOD、GSH-Px)显著升高。实时荧光定量PCR结果表明,Se-DD98高剂量组肝脏中Sod1(3.58)和Gpx1(7.14)表达显著上调,分别上调16.41、10.19倍,促炎因子IL-1β表达(2.16)显著下调1.61倍,抑炎因子IL-10(7.09)显著上调1.40倍。此外,Se-DD98高、低剂量组均能减轻X射线所致的肝脏损伤。最后,Se-DD98高剂量组各项指标与正常对照组相比差异不大,与辐射对照组差异显著。因此,富硒长双歧杆菌DD98可以通过减少氧化压力水平、增强抗氧化酶活力及抑制组织炎症来缓解X射线所致的小鼠放射性肝损伤。The purpose of this study was to examine the protective effects of selenium-enriched Bifidobacterium longum DD98(Se-DD98)on X-ray-induced hepatic damage in mice were investigated.Male SPF-grade balb/c mice were randomly divided into 5 groups:normal control group,radiation control group,Se-DD98 high-dose group,Se-DD98 low-dose group and DD98 control group.After 28 days of administration,the body weights of mice were monitored.Whole body X-ray irradiation was used on the 29th day,and then relevant indicators were measured after the experiment.Compared with the body weight(24.44 g)and liver index(4.49%)of the radiation control group,the body weight(26.96 g)and liver index(5.10%)of the Se-DD98 high-dose group increased significantly,with the levels of their serum liver function indicators(ALT,AST)and body oxidative stress indicators(MDA,LDH)decreasing significantly,and the activities of their antioxidant enzymes(CAT,SOD,GSH-Px)increasing significantly.Real-time fluorescent quantitative PCR results showed that the expression of Sod1(3.58)and Gpx1(7.14)in the liver of the Se-DD98 high-dose group was significantly up-regulated(by 16.41 and 10.19 times,respectively),and the expression of pro-inflammatory factor IL-1β(2.16)was significantly down-regulated(by 1.61 times).The mRNA levels of hepatic Sod1(3.58)and Gpx1(7.14)were significantly up-regulated respectively by 16.41 and 10.19 times in Se-DD98 high-dose group.In addition,both the high-and low-dose Se-DD98 groups could reduce X-ray-induced liver damage.Finally,the indices of the Se-DD98 high-dose group differed very little from the normal control group but differed significantly from the radiation control group.Therefore,Se-DD98 can alleviate X-ray-induced hepatic damage in mice through reducing the level of oxidative stress,boosting the activities of the antioxidant enzymes and inhibiting tissue inflammation.

关 键 词：X射线 肝损伤 氧化应激 抗氧化 炎症 富硒长双歧杆菌DD98 

分 类 号：TS201.3[轻工技术与工程—食品科学] R818.7[轻工技术与工程—食品科学与工程]