深层发酵用高产灵芝胞内多糖菌株的筛选  

Screening of Ganoderma lucidum Strains for High Intracellular Polysaccharide Yield in Submerged Fermentation

作　　者：李娜[1,2] 唐传红 刘艳芳[2] 周帅[2] 谭贻[2] 王金艳 冯杰[2] 张劲松[2] LI Na;TANG Chuanhong;LIU Yanfang;ZHOU Shuai;TAN Yi;WANG Jinyan;FENG Jie;ZHANG Jingsong(College of Food Science&Technology,Shanghai Ocean University,Shanghai 201306,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungal Processing,Shanghai 201403,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海201403

出　　处：《食用菌学报》2021年第3期63-71,共9页Acta Edulis Fungi

基　　金：上海市科技兴农重点攻关项目[沪农科创字(2018)第1-1号]。

摘　　要：对41个杂交灵芝(Ganoderma lucidum)菌株进行深层发酵培养,评价其菌丝体干重、胞内多糖含量和得率,及胞内多糖分子量分布特征和刺激巨噬细胞释放NO的活性,结果表明:杂交菌株的菌丝体干重、胞内多糖含量和得率与亲本相比有不同程度的差异,与两个亲本相比,菌丝体干重提高的有29个菌株,胞内多糖含量显著增加的有3个菌株,胞内多糖得率显著提高的有7个菌株;不同菌株胞内多糖均主要含有两个组分,且含量差异较大;不同菌株的胞内多糖在200μg·mL^(-1)时均有明显的刺激巨噬细胞释放NO的活性。综合考虑最终筛选出深层发酵用菌株GZ02,其胞内多糖得率最高,为1.34 g·L^(-1)比亲本G0130和G0154分别提高120%和103%,在50 L发酵罐中对GZ02进行小试,发酵168 h后,菌丝体干重达23.14 g·L^(-1),胞内多糖得率达1.43 g·L^(-1)。Through comparison on dry mycelium weight,intracellular polysaccharide content,and intracellular polysaccharide yield,eight hybrid Ganoderma lucidum strains were selected out of 41 candidates.The selected strains were further studied for molecular weight distribution characteristics of intracellular polysaccharides and the effect of them on NO release in macrophage.The results showed that compared with the parental strains,29 of the 41 candidate hybrid strains showed a greater dry mycelium weight,three hybrid strains had a greater intracellular polysaccharide content and seven hybrid strains had a greater intracellular polysaccharide yield.There are two fractions with large content variations in the intracellular polysaccharides of the eight selected hybrid strains.When used at 200μg·mL^(-1),the intracellular polysaccharides of the selected strains stimulated NO release in macrophage significantly.After a comprehensive evaluation,the hybrid strain GZ02 was selected for submerged fermentation in a 50 L bioreactor.The intracellular polysaccharide yield of GZ02 in shake flask cultures was 1.34 g·L^(-1),which was 120%and 103%greater than that of the parental strains G0130 and G0154,respectively.Upon fermentation in the 50 L bioreactor for 168 h,GZ02 yielded 23.14 g·L^(-1)mycelium(dry weight)and 1.43 g·L^(-1)intracellular polysaccharides.

关 键 词：灵芝 深层发酵 菌丝体 胞内多糖 

分 类 号：S567.31[农业科学—中草药栽培]