Co-cultured adventitious roots of Echinacea pallida and Echinacea purpurea inhibit lipopolysaccharide-induced inflammation via MAPK pathway in mouse peritoneal macrophages  

作　　者：Ming-zhi Fan Xiao-han Wu Xue-feng Li Xuan-chun Piao Jun Jiang Mei-lan Lian 

机构地区：[1]College of Agriculture,Yanbian University,Yanji 133002,China

出　　处：《Chinese Herbal Medicines》2021年第2期228-234,共7页中草药（英文版）

基　　金：supported by grants the Jilin Scientific and Technological Development Program (20180101278JC) for the financial support;the National Natural Science Foundation of China (31370388 and 31660080)。

摘　　要：Objective:In order to elucidate the biological activity of the Co-cultured adventitious roots(ARs) of Echinacea pallida and Echinacea purpurea and provide theoretical basis for its application,and the antiinflammatory activities and potential mechanisms of Co-cultured ARs were studied.Methods:The experimental materials were obtained by bioreactor co-culture technology and used in the activity research.In this study,mouse macrophages induced by lipopolysaccharide(LPS) were used as in vitro model.Different concentrations of AR extract(50-400 g/mL) were used to treat cells.The expression of pro-inflammatory cytokines was determined using enzyme linked immunosorbent assay.The inducible nitric oxide synthase and cyclooxygenase-2 expression,mitogen-activated protein kinase(MAPK) phosphorylation,and the inhibitor of nuclear factor-kappa B-a levels were determined by the Western blot analysis.Results:In the co-cultured ARs,total flavonoids and total caffeic acid were determined,and the contents of both bioactive compounds were significantly higher than those ARs from the single-species culture.Compared with the control group,the large amount of pro-inflammatory mediators was released after LPS stimulation.However,in the extract groups with different concentrations(25,50,and 100 g/mL),the production of these pro-inflammatory mediators was inhibited in a dose-dependent manner.Furthermore,the levels of phosphorylation of MAPK proteins,including p-p38, p-c-Jun N-terminal kinase,and p-extracellular regulated protein kinases were significantly(P <0.05) decreased in the extract groups,revealing that the AR extract probably involved in regulating the MAPK signaling pathway.Conclusion:Collectively,our findings suggested that the co-cultured ARs of E.pallida and E.purpurea can inhibit production of pro-inflammatory mediators in mouse peritoneal macrophages and possess the anti-inflammatory effect by regulating MAPK signaling pathways.

关 键 词：adventitious root ANTI-INFLAMMATION CO-CULTURE Echinacea pallida(Nutt.)Nutt Echinacea purpurea(L.) Moench mitogen-activated protein kinase 

分 类 号：R285.5[医药卫生—中药学]