麻痹性贝类毒素GTX1/4和GTX2/3的提取与纯化  

Extraction and purification of paralytic shellfish toxins GTX1/4 and GTX2/3

作　　者：金薇 姚敬元 贾宝林 梁玉波[2,3] 吕颂辉 JIN Wei;YAO Jing-yuan;JIA Bao-lin;LIANG Yu-bo;LV Song-hui(Jinan University,Guangzhou 510632,China;National Marine Environmental Monitoring Center,Dalian 116023,China;Dalian key laboratory of algal toxin,Dalian 116023,China)

机构地区：[1]暨南大学,广东广州510632 [2]国家海洋环境监测中心,辽宁大连116023 [3]大连市藻毒素重点实验室,辽宁大连116023

出　　处：《海洋环境科学》2021年第3期425-429,共5页Marine Environmental Science

基　　金：国家重点研发计划项目(2016YFF0201104,2017FYC1404303);国家自然科学基金项目(41576120,41276099);国家海洋局海洋赤潮灾害立体监测技术与应用重点实验室开放基金项目(MAYTHAB201605);国家海洋局近岸海域生态环境重点实验室开发基金项目(201613)。

摘　　要：麻痹性贝类毒素GTX1/4和GTX2/3是我国近海危害最严重的藻毒素。本研究以染毒紫贻贝为原料,贝肉均质后以0.18 mol/L甲酸溶液超声提取,提取液先用乙酸乙酯和氯仿洗涤,然后用大孔吸附树脂SP700净化,亲水型高效制备液相色谱分离纯化,得到麻痹性贝类毒素GTX1/4和GTX2/3组分,为麻痹性贝类毒素标准物质的研制提供了可靠的技术方法。GTX1/4 and GTX2/3 are the most harmful algal toxins in the coastal waters of China. The paralytic shellfish toxins from mussel samples were extracted with 0.18 mol/L formic acid aqueous solution, cleaned up with ethyl acetate and trichloromethane. Then, the crude PSTs solutions were separated by macroporous adsorption resin SP700 and purified by preparative high performance liquid chromatographic(PHPLC) coupled with Hillic column. It developed an effective method for the preparation of paralytic shellfish toxins reference materials.

关 键 词：麻痹性贝类毒素 膝沟藻毒素 贻贝 提取 纯化 

分 类 号：X171.5[环境科学与工程—环境科学]