利用甲酸产单细胞蛋白菌株的筛选及发酵工艺  

Screening and fermentation process of single cell protein-producing strains by formic acid

作　　者：赵立枝 朱大玲[1] 李德茂[2,3] ZHAO Li-zhi;ZHU Da-ling;LI De-mao

机构地区：[1]天津科技大学天津市卤水化工与资源生态化利用重点实验室,天津300457 [2]中国科学院天津工业生物技术研究所天津市工业生物系统与过程工程重点实验室,天津300308 [3]国家合成生物技术创新中心,天津300308

出　　处：《饲料研究》2021年第9期88-93,共6页Feed Research

基　　金：国家重点研发计划(项目编号:2018YFA0901500);天津市合成生物提升行动计划项目(项目编号:TSBICIP-KJGG-004-02);天津市卤水化工与资源生态化利用重点实验室开放基金项目(项目编号:BCERE201908)。

摘　　要：研究旨在筛选分离能够转化甲酸生产蛋白的高效菌株,并对其生理特性和发酵工艺进行研究。试验以5 g/L甲酸钠为碳源的培养基为筛选培养基,以生物量、甲酸利用率、蛋白含量为评价参数筛选获得高效菌株MA5。经16S rDNA序列分析鉴定,命名为副球菌Paracoccus communis MA5。采用单因素分析法对菌株的生理性能进行分析,发现菌株MA5最佳生长温度37℃、初始pH值7、甲酸钠浓度5 g/L和氮源8 g/L酵母粉。采用3 L发酵系统对菌株MA5的发酵工艺进行分析,发现发酵过程中菌株的甲酸消耗速率最高达0.77 g/(L·h)。在发酵末期菌株生物量干重达4.67 g/L,蛋白含量为37%。因此,副球菌MA5能够高效转化甲酸产单细胞蛋白。The study aimed to screen and isolate efficient strains that can use formic acid to produce single-cell protein,and study its physiological characteristics and fermentation process.In the study,the medium with 5 g/L sodium formate as carbon source was used as screening medium,and the efficient strain MA5 was screened and obtained by the evaluation parameters of biomass,formic acid utilization rate and protein content.The strain was identified and named Paracoccus communis MA5 by 16S rDNA sequence analysis.The physiological property of the strain were analyzed by the single factor method,and the results showed that the optimum growth temperature was 37℃,initial pH value was 5,sodium formate concentration was 5 g/L,and nitrogen source of strain MA5 was 8 g/L yeast extract powder.The fermentation process of strain MA5 was analyzed by the 3 L fermentation system.The results showed that the consumption rate of formate reached up to 0.77 g/(L·h) in the fermentation process,the dry weight of biomass reached 4.67 g/L and the protein content was 37% at the end of fermentation.Therefore,P.communis MA5 can efficiently transform formate to produce single cell protein.

关 键 词：甲酸 单细胞蛋白 培养基优化 副球菌 饲料 发酵 

分 类 号：S816[农业科学—饲料科学]