辣木叶粗多糖的制备·单糖组成及抗氧化活性研究  

Study on Preparation,Monosaccharide Composition and Antioxidant Activity of Crude Polysaccharide from Moringa oleifera Leaves

作　　者：聂烁 吴佳怡 程乃雪 闻正顺 王文策[2] NIE Shuo;WU Jia-yi;CHENG Nai-xue(School of Food Science and Pharmaceutics,Zhejiang Ocean University,Zhoushan,Zhejiang 316022)

机构地区：[1]浙江海洋大学食品与药学学院,浙江舟山316022 [2]华南农业大学动物科学学院,广东广州510642

出　　处：《安徽农业科学》2021年第12期183-186,共4页Journal of Anhui Agricultural Sciences

基　　金：浙江省属高校基本科研业务费(2019JZ00010);浙江省大学生科技创新项目(2017R411008);舟山市科技合作项目(2020C21014,2020C21047,2021C13044);浙江省重点研发项目(2019C02076)。

摘　　要：[目的]制备辣木叶粗多糖并研究其单糖组成和抗氧化活性。[方法]以华南地区辣木叶为原料,水提醇沉法、Sevage法、过氧化氢(H2O2)法提取辣木叶粗多糖,水解衍生化分析单糖组成,并通过DPPH自由基清除能力、羟基自由基清除能力、超氧阴离子自由基清除能力、还原力等指标评价辣木叶粗多糖体外抗氧化活性。[结果]该试验所制备的辣木叶粗多糖提取率为7.16%,测得纯度为84.33%。辣木叶粗多糖的单糖组成有甘露糖、鼠李糖、葡萄糖、半乳糖和木糖,各占56.88%、14.44%、10.54%、9.45%和4.13%。另外,试验表明辣木叶粗多糖具有良好的自由基清除能力,抗氧化活性较好且与浓度呈正相关。[结论]该研究为辣木叶多糖的深入研究和开发利用提供科学依据。[Objective]To prepare crude polysaccharides from Moringa oleifera leaves and to study its monosaccharide composition and antioxidant activity.[Method]Using Moringa oleifera leaves in South China as raw materials,the crude polysaccharides of Moringa oleifera leaves were extracted by water extraction and alcohol precipitation method,Sevage method,and hydrogen peroxide(H2O2)method.The anti-oxidant activity of Moringa oleifera leaves polysaccharide was evaluated by indicators such as DPPH free radical scavenging ability,hydroxyl free radical scavenging ability,superoxide anion free radical scavenging ability and reducing power.[Result]The extraction rate of crude polysaccharide from Moringa oleifera leaves prepared in this experiment was 7.16%,and the measured purity was 84.33%.Moringa oleifera leaves crude polysaccharides consist of mannose,rhamnose,glucose,galactose and xylose,which account for 56.88%,14.44%,10.54%,9.45%and 4.13%respectively.In addition,the experiment showed that the crude polysaccharide of Moringa oleifera leaves had good free radical scavenging ability,good antioxidant activity and positive correlation with concentration.[Conclusion]The study provides scientific basis for further research and development of Moringa oleifera leaves.

关 键 词：辣木叶 粗多糖 制备 单糖组成 抗氧化活性 

分 类 号：R285[医药卫生—中药学]