响应面法优化灵芝发酵三七渣产灵芝三萜工艺  

Optimization of fermentation conditions for Ganoderma lucidum triterpenoids production with notoginseng residues by Ganoderma lucidum using response surface methodology

作　　者：谭显东[1] 陈楠 王浪[1] 魏琨 吉栗漫 TAN Xiandong;CHEN Nan;WANG Lang;WEI Kun;JI Liman(College of Resources and Environment,Chengdu University of Information Technology,Chengdu 610225,China)

机构地区：[1]成都信息工程大学资源环境学院,四川成都610225

出　　处：《中国酿造》2021年第6期147-151,共5页China Brewing

基　　金：成都市重点研发支撑计划(2019-YF05-02457-SN);四川省重点研发项目(2021YFG0263)。

摘　　要：通过单因素试验和Box-Behnken试验对三七渣固态发酵生产灵芝三萜的发酵条件进行优化。结果表明,各影响因素对于发酵培养物中灵芝三萜含量影响程度的排序为:培养基初始含水量>酵母粉添加量>磷酸盐添加量。优化发酵条件为:过60目筛的三七渣,酵母浸出粉添加量2.5%,磷酸二氢钾添加量0.15%,培养基初始含水量65%。在此优化条件下,发酵培养物中灵芝三萜含量为0.37%。The solid-state fermentation conditions of notoginseng residues for the preparation of Ganoderma lucidum triterpenoids were optimized by single factor experiments and Box-Behnken experiments.The results showed that the effect of various factors on the content of G.lucidum triterpenoids in fermentation culture in order was initial water content of the medium>yeast powder addition>potassium dihydrogen phosphate addition.The optimized fermentation conditions were obtained as follows:notoginseng residues crushed and sieved to 60 mesh,yeast extract powder 2.5%,potassium dihydrogen phosphate 0.15%,initial medium moisture 65%.Under the optimal conditions,the content of G.lucidum triterpenoid in the culture medium was 0.37%.

关 键 词：三七渣 灵芝三萜 发酵 响应面法 

分 类 号：R284.2[医药卫生—中药学]